Detailed information of TA system TA101123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 145672..146342
• Replicon: plasmid pVir_095132
• Accession: NZ_CP028390
• Organism: Klebsiella pneumoniae strain WCHKP13F2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: B7D34_RS01960
• Protein ID: WP_004213072.1
• Coordinates: 145672..146115 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: B7D34_RS01965
• Protein ID: WP_004213073.1
• Coordinates: 146112..146342 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D34_RS01915	141080..141355	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
B7D34_RS01920	141418..141909	+	492	WP_011251327.1	DM13 domain-containing protein	-
B7D34_RS01925	141958..142878	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
B7D34_RS01930	142969..143172	+	204	WP_004213936.1	hypothetical protein	-
B7D34_RS01935	143100..143375	+	276	WP_004213938.1	GAF domain-containing protein	-
B7D34_RS01940	143440..143633	-	194	Protein_156	hypothetical protein	-
B7D34_RS01945	144184..144528	-	345	Protein_157	hypothetical protein	-
B7D34_RS01950	144945..145238	+	294	Protein_158	IS3 family transposase	-
B7D34_RS01955	145242..145658	+	417	Protein_159	DUF3644 domain-containing protein	-
B7D34_RS01960	145672..146115	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D34_RS01965	146112..146342	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B7D34_RS01970	146950..148083	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
B7D34_RS01975	148099..148392	+	294	WP_004213076.1	hypothetical protein	-
B7D34_RS01980	148382..148588	-	207	WP_004213077.1	hypothetical protein	-
B7D34_RS01985	148940..149230	+	291	WP_004213078.1	hypothetical protein	-
B7D34_RS01990	149220..150119	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2	1..208166	208166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T101123 WP_004213072.1 NZ_CP028390:c146115-145672 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T101123 NZ_CP028390:c146115-145672 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT101123 WP_004213073.1 NZ_CP028390:c146342-146112 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT101123 NZ_CP028390:c146342-146112 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620