Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 145672..146342 | Replicon | plasmid pVir_095132 |
| Accession | NZ_CP028390 | ||
| Organism | Klebsiella pneumoniae strain WCHKP13F2 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q6U619 |
| Locus tag | B7D34_RS01960 | Protein ID | WP_004213072.1 |
| Coordinates | 145672..146115 (-) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q6U620 |
| Locus tag | B7D34_RS01965 | Protein ID | WP_004213073.1 |
| Coordinates | 146112..146342 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B7D34_RS01915 | 141080..141355 | + | 276 | WP_004213932.1 | carboxymuconolactone decarboxylase family protein | - |
| B7D34_RS01920 | 141418..141909 | + | 492 | WP_011251327.1 | DM13 domain-containing protein | - |
| B7D34_RS01925 | 141958..142878 | + | 921 | WP_004213934.1 | DUF1471 domain-containing protein | - |
| B7D34_RS01930 | 142969..143172 | + | 204 | WP_004213936.1 | hypothetical protein | - |
| B7D34_RS01935 | 143100..143375 | + | 276 | WP_004213938.1 | GAF domain-containing protein | - |
| B7D34_RS01940 | 143440..143633 | - | 194 | Protein_156 | hypothetical protein | - |
| B7D34_RS01945 | 144184..144528 | - | 345 | Protein_157 | hypothetical protein | - |
| B7D34_RS01950 | 144945..145238 | + | 294 | Protein_158 | IS3 family transposase | - |
| B7D34_RS01955 | 145242..145658 | + | 417 | Protein_159 | DUF3644 domain-containing protein | - |
| B7D34_RS01960 | 145672..146115 | - | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| B7D34_RS01965 | 146112..146342 | - | 231 | WP_004213073.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| B7D34_RS01970 | 146950..148083 | + | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| B7D34_RS01975 | 148099..148392 | + | 294 | WP_004213076.1 | hypothetical protein | - |
| B7D34_RS01980 | 148382..148588 | - | 207 | WP_004213077.1 | hypothetical protein | - |
| B7D34_RS01985 | 148940..149230 | + | 291 | WP_004213078.1 | hypothetical protein | - |
| B7D34_RS01990 | 149220..150119 | + | 900 | WP_004225022.1 | nucleotide-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 | 1..208166 | 208166 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 16006.74 Da Isoelectric Point: 9.3642
>T101123 WP_004213072.1 NZ_CP028390:c146115-145672 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
Download Length: 444 bp
>T101123 NZ_CP028390:c146115-145672 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.73 Da Isoelectric Point: 4.4563
>AT101123 WP_004213073.1 NZ_CP028390:c146342-146112 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT101123 NZ_CP028390:c146342-146112 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|