Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 52064..52800 Replicon plasmid pVir_095132
Accession NZ_CP028390
Organism Klebsiella pneumoniae strain WCHKP13F2

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag B7D34_RS01415 Protein ID WP_004098919.1
Coordinates 52318..52800 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag B7D34_RS01410 Protein ID WP_004213599.1
Coordinates 52064..52330 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D34_RS01385 47296..47853 + 558 WP_004213590.1 recombinase family protein -
B7D34_RS01390 47856..50825 + 2970 WP_004213592.1 Tn3 family transposase -
B7D34_RS01395 50867..51361 + 495 WP_004213594.1 hypothetical protein -
B7D34_RS01400 51422..51625 + 204 WP_004213596.1 hemolysin expression modulator Hha -
B7D34_RS01405 51639..51869 + 231 WP_004213598.1 hypothetical protein -
B7D34_RS01410 52064..52330 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
B7D34_RS01415 52318..52800 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
B7D34_RS01420 53176..54448 + 1273 Protein_52 IS3 family transposase -
B7D34_RS01425 54444..54839 - 396 Protein_53 IS3 family transposase -
B7D34_RS01430 54814..55017 - 204 WP_011154554.1 hypothetical protein -
B7D34_RS01435 55014..56036 - 1023 WP_004214536.1 porphobilinogen synthase -
B7D34_RS01440 56100..57446 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 1..208166 208166
- inside IScluster/Tn - - 47296..54448 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T101121 WP_004098919.1 NZ_CP028390:52318-52800 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T101121 NZ_CP028390:52318-52800 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT101121 WP_004213599.1 NZ_CP028390:52064-52330 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT101121 NZ_CP028390:52064-52330 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References