Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5880..6405 Replicon plasmid pKPC2_095132
Accession NZ_CP028389
Organism Klebsiella pneumoniae strain WCHKP13F2

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag B7D34_RS00040 Protein ID WP_013023785.1
Coordinates 5880..6185 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag B7D34_RS00045 Protein ID WP_001568025.1
Coordinates 6187..6405 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D34_RS00010 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
B7D34_RS00015 2313..2492 + 180 WP_006788217.1 hypothetical protein -
B7D34_RS00020 2956..3750 - 795 WP_004197635.1 site-specific integrase -
B7D34_RS00025 3948..4964 - 1017 WP_017899884.1 hypothetical protein -
B7D34_RS00030 4975..5289 - 315 WP_053389906.1 hypothetical protein -
B7D34_RS00035 5316..5675 - 360 WP_025380813.1 hypothetical protein -
B7D34_RS00040 5880..6185 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
B7D34_RS00045 6187..6405 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
B7D34_RS00050 6457..6651 + 195 WP_071571078.1 hypothetical protein -
B7D34_RS00055 6575..6913 - 339 WP_100792567.1 hypothetical protein -
B7D34_RS00060 7036..7233 + 198 WP_017899886.1 hypothetical protein -
B7D34_RS00065 7223..7513 - 291 WP_013023783.1 hypothetical protein -
B7D34_RS00070 7510..8637 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
B7D34_RS00075 8671..10263 - 1593 WP_015344964.1 hypothetical protein -
B7D34_RS00080 10470..11249 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / aph(3')-Ia / mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) / floR / blaKPC-2 - 1..166034 166034


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T101120 WP_013023785.1 NZ_CP028389:c6185-5880 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T101120 NZ_CP028389:c6185-5880 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT101120 WP_001568025.1 NZ_CP028389:c6405-6187 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT101120 NZ_CP028389:c6405-6187 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References