Detailed information of TA system TA101118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 142817..143525
• Replicon: plasmid unnamed
• Accession: NZ_CP028385
• Organism: Providencia heimbachae strain 99101

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C9446_RS20680
• Protein ID: WP_137022559.1
• Coordinates: 143136..143525 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7IVZ1
• Locus tag: C9446_RS20675
• Protein ID: WP_067421833.1
• Coordinates: 142817..143146 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9446_RS20650	138554..138835	+	282	WP_137022554.1	hypothetical protein	-
C9446_RS20655	138919..140112	+	1194	WP_137022555.1	hypothetical protein	-
C9446_RS20660	140321..140872	+	552	WP_137022556.1	hypothetical protein	-
C9446_RS20665	140960..142609	+	1650	WP_137022557.1	ATP-binding protein	-
C9446_RS20670	142596..142778	+	183	WP_137022558.1	hypothetical protein	-
C9446_RS20675	142817..143146	-	330	WP_067421833.1	helix-turn-helix domain-containing protein	Antitoxin
C9446_RS20680	143136..143525	-	390	WP_137022559.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9446_RS20685	143668..144276	+	609	WP_137022560.1	hypothetical protein	-
C9446_RS20690	144330..145550	+	1221	WP_137022561.1	hypothetical protein	-
C9446_RS20695	145643..146089	+	447	WP_137022562.1	hypothetical protein	-
C9446_RS21515	146134..146274	+	141	WP_175403207.1	hypothetical protein	-
C9446_RS20700	146309..146623	+	315	WP_137022563.1	hypothetical protein	-
C9446_RS20705	146627..147415	+	789	WP_137022564.1	M48 family metallopeptidase	-
C9446_RS20710	147483..148160	+	678	WP_137022565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catB8 / blaOXA-10 / ant(3'')-Ia / dfrA1 / qacE / sul1	-	1..231957	231957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-93)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14450.56 Da
• Isoelectric Point: 8.4662

>T101118 WP_137022559.1 NZ_CP028385:c143525-143136 [Providencia heimbachae]
VAFYKISSFVRSTKKIPITDKQLLDAAHEVQQGQFEADLGGGVIKKRLAMKGQGKSGSVRVIIFFKINNHLFFADGWTKN
TVNAKGTKEIEDDELETYKKLAAEFLNFDGKIINNLISKGILEEITDEQ

Nucleotide

• Length: 390 bp

>T101118 NZ_CP028385:c143525-143136 [Providencia heimbachae]
GTGGCGTTCTATAAAATCTCATCTTTTGTCCGAAGCACCAAAAAAATACCTATAACAGACAAACAGTTACTTGATGCTGC
TCATGAAGTTCAACAAGGACAATTTGAAGCTGATTTAGGCGGTGGAGTGATAAAAAAACGCCTTGCAATGAAAGGGCAAG
GCAAAAGTGGAAGTGTTAGGGTTATTATCTTTTTTAAGATAAACAATCATCTTTTTTTTGCAGATGGATGGACCAAAAAC
ACGGTCAATGCCAAAGGTACAAAAGAGATTGAAGATGATGAGTTGGAAACCTATAAAAAATTAGCCGCTGAGTTTCTGAA
CTTTGATGGAAAAATCATTAACAATTTAATTTCTAAAGGAATTCTGGAGGAAATTACTGATGAACAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12062.15 Da
• Isoelectric Point: 10.2039

>AT101118 WP_067421833.1 NZ_CP028385:c143146-142817 [Providencia heimbachae]
MNNKLNNMLELAKELNAAGASPESLVKDIESTIRAREINDKIKEVSAMSGSEIKMLRTKYCMSQSVLARALGMSKESISK
WERGEKKPSGPALRMLRILESKGPEVLVV

Nucleotide

• Length: 330 bp

>AT101118 NZ_CP028385:c143146-142817 [Providencia heimbachae]
ATGAACAATAAACTTAATAACATGCTTGAATTGGCTAAAGAGTTAAATGCAGCTGGTGCATCACCTGAAAGCCTGGTTAA
AGATATTGAAAGCACCATCCGTGCTCGTGAAATTAACGATAAAATAAAAGAAGTTTCAGCGATGAGCGGCTCTGAGATCA
AGATGTTGCGGACTAAATATTGTATGTCTCAATCTGTGCTCGCTCGTGCATTGGGAATGTCGAAAGAGAGCATATCCAAA
TGGGAGCGAGGAGAAAAGAAACCAAGCGGCCCTGCATTGAGGATGCTACGTATTCTTGAAAGCAAAGGCCCAGAAGTTTT
AGTGGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A4D7IVZ1