Detailed information of TA system TA101116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 4184732..4185239
• Replicon: chromosome
• Accession: NZ_CP028384
• Organism: Providencia heimbachae strain 99101

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7JI57
• Locus tag: C9446_RS19500
• Protein ID: WP_068438114.1
• Coordinates: 4184979..4185239 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: C9446_RS19495
• Protein ID: WP_137022342.1
• Coordinates: 4184732..4184986 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9446_RS19475	4180399..4180827	+	429	WP_137022338.1	protein tyrosine phosphatase	-
C9446_RS19480	4180862..4182940	+	2079	WP_137022339.1	polysaccharide biosynthesis tyrosine autokinase	-
C9446_RS19485	4182958..4183983	+	1026	WP_137022340.1	UDP-glucose 4-epimerase GalE	-
C9446_RS19490	4184143..4184646	+	504	WP_137022341.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
C9446_RS19495	4184732..4184986	+	255	WP_137022342.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C9446_RS19500	4184979..4185239	+	261	WP_068438114.1	Txe/YoeB family addiction module toxin	Toxin
C9446_RS19505	4185254..4186075	-	822	WP_137022343.1	serine O-acetyltransferase	-
C9446_RS19510	4186085..4187118	-	1034	Protein_3811	NAD(P)H-dependent glycerol-3-phosphate dehydrogenase	-
C9446_RS19515	4187118..4187594	-	477	WP_068438107.1	protein-export chaperone SecB	-
C9446_RS19520	4187666..4188103	-	438	WP_068438104.1	rhodanese-like domain-containing protein	-
C9446_RS19525	4188537..4189829	+	1293	WP_137022344.1	murein hydrolase activator EnvC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10422.83 Da
• Isoelectric Point: 8.8207

>T101116 WP_068438114.1 NZ_CP028384:4184979-4185239 [Providencia heimbachae]
MSRFLAWTDQAWEDYLYWQTQDKKTLKRINKLIDDTKRTPFYGIGKPEPLKENLAGFWSRRIDETNRLIYSVDDTNLTIV
ACRYHY

Nucleotide

• Length: 261 bp

>T101116 NZ_CP028384:4184979-4185239 [Providencia heimbachae]
ATGAGTAGGTTTTTAGCATGGACTGATCAAGCATGGGAAGACTACCTATATTGGCAAACTCAAGACAAAAAAACTCTCAA
ACGTATTAATAAGCTTATCGATGATACTAAAAGAACACCATTTTACGGAATAGGAAAGCCAGAGCCACTAAAAGAGAATT
TAGCTGGCTTCTGGTCTCGTAGAATAGATGAAACAAATAGGCTTATCTATTCAGTAGATGATACAAATTTAACTATTGTT
GCTTGTCGTTATCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9562.90 Da
• Isoelectric Point: 7.0113

>AT101116 WP_137022342.1 NZ_CP028384:4184732-4184986 [Providencia heimbachae]
MKTISFSDARNNLKAVLDRVIDDADTTIITRRDSEDAVVMSLEYYNSLMETIYLLRSPANAEHLNKSIAQFKAGKAKKRE
LINE

Nucleotide

• Length: 255 bp

>AT101116 NZ_CP028384:4184732-4184986 [Providencia heimbachae]
ATGAAAACTATTTCTTTCAGTGATGCTAGAAACAATCTAAAAGCAGTATTAGATAGAGTTATTGATGACGCAGATACAAC
TATCATCACTCGTCGTGACTCAGAAGATGCTGTAGTGATGTCACTGGAATATTATAATAGCCTTATGGAAACGATTTATT
TATTACGTTCTCCAGCAAATGCCGAACATTTAAATAAATCCATCGCTCAATTTAAAGCAGGTAAAGCGAAAAAAAGAGAG
CTGATAAATGAGTAG

Structures

Toxin

• AlphaFold DB: A0A4D7JI57