Detailed information of TA system TA101102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 20278..20825
• Replicon: plasmid pRM10466-2
• Accession: NZ_CP028383
• Organism: Escherichia coli strain RM10466

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C7R96_RS27530
• Protein ID: WP_000970004.1
• Coordinates: 20544..20825 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: W0FTF6
• Locus tag: C7R96_RS27525
• Protein ID: WP_000079942.1
• Coordinates: 20278..20547 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7R96_RS27475	15338..15541	+	204	WP_001336517.1	hypothetical protein	-
C7R96_RS27480	16286..16747	+	462	WP_000760088.1	thermonuclease family protein	-
C7R96_RS27485	16850..17233	+	384	WP_001109063.1	hypothetical protein	-
-	17408..17464	-	57	NuclAT_1	-	-
-	17408..17464	-	57	NuclAT_1	-	-
-	17408..17464	-	57	NuclAT_1	-	-
-	17408..17464	-	57	NuclAT_1	-	-
C7R96_RS27495	17456..17662	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	-
C7R96_RS27500	17946..18194	+	249	WP_000083838.1	replication regulatory protein RepA	-
C7R96_RS27510	18441..18515	+	75	WP_001365571.1	RepA leader peptide Tap	-
C7R96_RS27515	18508..19365	+	858	WP_000130991.1	incFII family plasmid replication initiator RepA	-
C7R96_RS27525	20278..20547	+	270	WP_000079942.1	hypothetical protein	Antitoxin
C7R96_RS27530	20544..20825	+	282	WP_000970004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7R96_RS28265	21262..21498	+	237	WP_032257624.1	colicin V immunity protein	-
C7R96_RS27540	21476..21787	+	312	WP_001259758.1	colicin V	-
C7R96_RS27545	21957..24071	-	2115	WP_109996808.1	peptidase domain-containing ABC transporter	-
C7R96_RS27550	24046..25320	-	1275	WP_021573925.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67750	67750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10846.54 Da
• Isoelectric Point: 8.4615

>T101102 WP_000970004.1 NZ_CP028383:20544-20825 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLVAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T101102 NZ_CP028383:20544-20825 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGGT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCTGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10282.61 Da
• Isoelectric Point: 7.2057

>AT101102 WP_000079942.1 NZ_CP028383:20278-20547 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT101102 NZ_CP028383:20278-20547 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A140JYS5