Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 96701..97226 Replicon plasmid pRM10466-1
Accession NZ_CP028382
Organism Escherichia coli strain RM10466

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C7R96_RS27060 Protein ID WP_001159871.1
Coordinates 96701..97006 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C7R96_RS27065 Protein ID WP_000813639.1
Coordinates 97008..97226 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7R96_RS27030 92696..92869 + 174 Protein_98 DUF4113 domain-containing protein -
C7R96_RS27035 92871..93287 - 417 WP_001278809.1 plasmid partitioning/stability family protein -
C7R96_RS27040 93280..94260 - 981 WP_000688508.1 plasmid segregation protein ParM -
C7R96_RS27045 94675..94983 - 309 WP_000030199.1 hypothetical protein -
C7R96_RS27050 95070..95714 - 645 WP_001144036.1 ParA family protein -
C7R96_RS27055 95894..96700 - 807 WP_000016966.1 site-specific integrase -
C7R96_RS27060 96701..97006 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C7R96_RS27065 97008..97226 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C7R96_RS27070 97822..98082 + 261 WP_000343100.1 hypothetical protein -
C7R96_RS27075 98079..98669 + 591 WP_000194569.1 hypothetical protein -
C7R96_RS27080 98687..99034 - 348 WP_000142426.1 hypothetical protein -
C7R96_RS27090 99504..99839 - 336 Protein_109 colicin -
C7R96_RS27095 99934..100665 - 732 WP_000850413.1 complement resistance protein TraT -
C7R96_RS28245 100942..101052 - 111 Protein_111 IS481 family transposase -
C7R96_RS28420 101056..101267 - 212 Protein_112 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..160675 160675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T101101 WP_001159871.1 NZ_CP028382:c97006-96701 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T101101 NZ_CP028382:c97006-96701 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT101101 WP_000813639.1 NZ_CP028382:c97226-97008 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT101101 NZ_CP028382:c97226-97008 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References