Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4772481..4772978 Replicon chromosome
Accession NZ_CP028381
Organism Escherichia coli strain RM10466

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag C7R96_RS25060 Protein ID WP_001173344.1
Coordinates 4772805..4772978 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C7R96_RS25055 Protein ID WP_000398619.1
Coordinates 4772481..4772777 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7R96_RS25030 4769212..4769931 - 720 WP_000611288.1 two-component system response regulator DcuR -
C7R96_RS25035 4769928..4771559 - 1632 WP_001216461.1 sensor histidine kinase -
C7R96_RS25040 4771740..4771970 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
C7R96_RS25045 4771982..4772254 + 273 WP_000405647.1 N-acetyltransferase -
C7R96_RS25055 4772481..4772777 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C7R96_RS25060 4772805..4772978 + 174 WP_001173344.1 type V toxin-antitoxin system toxin GhoT Toxin
C7R96_RS25065 4773097..4774614 - 1518 WP_001295074.1 lysine--tRNA ligase -
C7R96_RS25070 4774851..4776308 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6583.12 Da        Isoelectric Point: 10.2288

>T101099 WP_001173344.1 NZ_CP028381:4772805-4772978 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSVFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T101099 NZ_CP028381:4772805-4772978 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGTATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT101099 WP_000398619.1 NZ_CP028381:4772481-4772777 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT101099 NZ_CP028381:4772481-4772777 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References