Detailed information of TA system TA101092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2877474..2878099
• Replicon: chromosome
• Accession: NZ_CP028381
• Organism: Escherichia coli strain RM10466

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7R96_RS15315
• Protein ID: WP_000911330.1
• Coordinates: 2877474..2877872 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C7R96_RS15320
• Protein ID: WP_000450524.1
• Coordinates: 2877872..2878099 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7R96_RS15295	2873352..2873552	+	201	WP_000383836.1	YpfN family protein	-
C7R96_RS15300	2873662..2874360	-	699	WP_000679807.1	esterase	-
C7R96_RS15305	2874434..2876449	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
C7R96_RS15310	2876464..2877327	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
C7R96_RS15315	2877474..2877872	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C7R96_RS15320	2877872..2878099	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C7R96_RS15325	2878253..2878966	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C7R96_RS15330	2879179..2880213	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
C7R96_RS15335	2880230..2881108	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C7R96_RS15340	2881254..2881826	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
C7R96_RS15345	2881826..2882296	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T101092 WP_000911330.1 NZ_CP028381:c2877872-2877474 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T101092 NZ_CP028381:c2877872-2877474 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT101092 WP_000450524.1 NZ_CP028381:c2878099-2877872 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT101092 NZ_CP028381:c2878099-2877872 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33