Detailed information of TA system TA101081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1317973..1318452
• Replicon: chromosome
• Accession: NZ_CP028381
• Organism: Escherichia coli strain RM10466

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0P7S0Y8
• Locus tag: C7R96_RS06825
• Protein ID: WP_032211256.1
• Coordinates: 1317973..1318260 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0P7PDW2
• Locus tag: C7R96_RS06830
• Protein ID: WP_001367155.1
• Coordinates: 1318261..1318452 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7R96_RS06785	1313144..1313413	-	270	WP_000003742.1	excisionase	-
C7R96_RS06790	1313475..1315919	-	2445	WP_087613937.1	exonuclease	-
C7R96_RS06795	1316012..1316200	-	189	WP_000092784.1	DUF1482 family protein	-
C7R96_RS06800	1316197..1316385	-	189	WP_000450222.1	cell division inhibitor	-
C7R96_RS06815	1316914..1317429	+	516	WP_000559919.1	hypothetical protein	-
C7R96_RS06820	1317543..1317695	-	153	WP_000380318.1	DUF1391 family protein	-
C7R96_RS06825	1317973..1318260	-	288	WP_032211256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7R96_RS06830	1318261..1318452	-	192	WP_001367155.1	hypothetical protein	Antitoxin
C7R96_RS06835	1318421..1318840	-	420	WP_087614071.1	helix-turn-helix domain-containing protein	-
C7R96_RS06840	1318901..1319272	-	372	WP_000448216.1	hypothetical protein	-
C7R96_RS06845	1319375..1319656	+	282	WP_021577226.1	helix-turn-helix domain-containing protein	-
C7R96_RS06850	1319660..1320085	+	426	WP_000693827.1	Rha family transcriptional regulator	-
C7R96_RS28015	1320154..1321185	+	1032	WP_052909572.1	phage replisome organizer	-
C7R96_RS06860	1321097..1321639	+	543	WP_072130322.1	replication protein	-
C7R96_RS06865	1321673..1322389	+	717	WP_047082289.1	DUF1627 domain-containing protein	-
C7R96_RS06870	1322422..1322703	+	282	WP_072094476.1	DNA-binding protein	-
C7R96_RS06875	1322700..1322981	+	282	WP_109996733.1	DUF4406 domain-containing protein	-
C7R96_RS06880	1323153..1323335	+	183	WP_022581296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	stx2A / stx2B	1309534..1330413	20879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11013.72 Da
• Isoelectric Point: 6.2118

>T101081 WP_032211256.1 NZ_CP028381:c1318260-1317973 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIE
IVNVVHARRQFPVTP

Nucleotide

• Length: 288 bp

>T101081 NZ_CP028381:c1318260-1317973 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACTTGGATGATATAACCAGCTATATTGCACGCTTTGACATAGA
TGCGGCAGAACGTTTATGGAAACGCTTAAGGACTTGCGTTCAGCCATTATCTGAGCATCCGTATTTATACCCGCCAAGTG
ACAGAGTGCCAGGTTTGCGTGAAATTGTAGCTCACCCCAATTACATTATTCTGTACCGAGTGACAATTTCAGGCATTGAA
ATTGTTAACGTAGTTCACGCCCGCCGACAGTTCCCTGTAACACCTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7365.28 Da
• Isoelectric Point: 5.1069

>AT101081 WP_001367155.1 NZ_CP028381:c1318452-1318261 [Escherichia coli]
MNTALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFAKMRKERSEQ

Nucleotide

• Length: 192 bp

>AT101081 NZ_CP028381:c1318452-1318261 [Escherichia coli]
ATGAACACAGCCCTTTCACCGATGGTTTCTGAATTTGAAACTGTCGAGCAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAGGTTGAGCGTAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGTGAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0P7S0Y8

Antitoxin

• AlphaFold DB: A0A0P7PDW2