Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5158480..5159075 Replicon chromosome
Accession NZ_CP028379
Organism Escherichia coli O145 str. RM9872

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag I3Y_RS26570 Protein ID WP_000239579.1
Coordinates 5158725..5159075 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag I3Y_RS26565 Protein ID WP_001223208.1
Coordinates 5158480..5158731 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Y_RS26555 5154145..5157924 + 3780 WP_000060846.1 autotransporter assembly complex protein TamB -
I3Y_RS26560 5157927..5158268 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
I3Y_RS26565 5158480..5158731 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
I3Y_RS26570 5158725..5159075 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
I3Y_RS26575 5159155..5159685 - 531 WP_000055075.1 inorganic diphosphatase -
I3Y_RS26580 5159995..5160951 + 957 WP_000265940.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
I3Y_RS26585 5161091..5162593 + 1503 WP_000205816.1 sugar ABC transporter ATP-binding protein -
I3Y_RS26590 5162607..5163629 + 1023 WP_001400707.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T101076 WP_000239579.1 NZ_CP028379:5158725-5159075 [Escherichia coli O145 str. RM9872]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T101076 NZ_CP028379:5158725-5159075 [Escherichia coli O145 str. RM9872]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT101076 WP_001223208.1 NZ_CP028379:5158480-5158731 [Escherichia coli O145 str. RM9872]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT101076 NZ_CP028379:5158480-5158731 [Escherichia coli O145 str. RM9872]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References