Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5019459..5019956 Replicon chromosome
Accession NZ_CP028379
Organism Escherichia coli O145 str. RM9872

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I3Y_RS25830 Protein ID WP_001173343.1
Coordinates 5019783..5019956 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I3Y_RS25825 Protein ID WP_000398619.1
Coordinates 5019459..5019755 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Y_RS25805 5016190..5016909 - 720 WP_000611288.1 two-component system response regulator DcuR -
I3Y_RS25810 5016906..5018537 - 1632 WP_022581427.1 sensor histidine kinase -
I3Y_RS25815 5018718..5018948 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
I3Y_RS25820 5018960..5019232 + 273 WP_000405653.1 N-acetyltransferase -
I3Y_RS25825 5019459..5019755 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I3Y_RS25830 5019783..5019956 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I3Y_RS25835 5020075..5021592 - 1518 WP_001295074.1 lysine--tRNA ligase -
I3Y_RS25840 5021829..5023286 - 1458 WP_000856822.1 dipeptide/tripeptide permease DtpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T101075 WP_001173343.1 NZ_CP028379:5019783-5019956 [Escherichia coli O145 str. RM9872]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T101075 NZ_CP028379:5019783-5019956 [Escherichia coli O145 str. RM9872]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT101075 WP_000398619.1 NZ_CP028379:5019459-5019755 [Escherichia coli O145 str. RM9872]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT101075 NZ_CP028379:5019459-5019755 [Escherichia coli O145 str. RM9872]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References