Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 484816..485434 Replicon chromosome
Accession NZ_CP028379
Organism Escherichia coli O145 str. RM9872

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I3Y_RS02295 Protein ID WP_001291435.1
Coordinates 484816..485034 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I3Y_RS02300 Protein ID WP_000344800.1
Coordinates 485060..485434 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Y_RS02260 480105..480677 + 573 WP_000779831.1 YbaY family lipoprotein -
I3Y_RS02265 480708..481019 - 312 WP_000409911.1 MGMT family protein -
I3Y_RS02275 481398..481751 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I3Y_RS02280 481793..483343 - 1551 WP_024221674.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I3Y_RS02285 483507..483977 - 471 WP_000136192.1 YlaC family protein -
I3Y_RS02290 484093..484644 - 552 WP_000102564.1 maltose O-acetyltransferase -
I3Y_RS02295 484816..485034 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I3Y_RS02300 485060..485434 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I3Y_RS02305 485980..489129 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I3Y_RS02310 489152..490345 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T101051 WP_001291435.1 NZ_CP028379:c485034-484816 [Escherichia coli O145 str. RM9872]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T101051 NZ_CP028379:c485034-484816 [Escherichia coli O145 str. RM9872]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT101051 WP_000344800.1 NZ_CP028379:c485434-485060 [Escherichia coli O145 str. RM9872]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT101051 NZ_CP028379:c485434-485060 [Escherichia coli O145 str. RM9872]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References