Detailed information of TA system TA101049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1246842..1247759
• Replicon: chromosome
• Accession: NZ_CP028375
• Organism: Bacillus velezensis strain W1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: C9888_RS06595
• Protein ID: WP_007407256.1
• Coordinates: 1247013..1247759 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: C9888_RS06590
• Protein ID: WP_014417527.1
• Coordinates: 1246842..1247012 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9888_RS06545	1242048..1242560	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
C9888_RS06550	1242673..1243332	+	660	WP_014417523.1	terminase	-
C9888_RS06555	1243230..1243697	+	468	Protein_1215	hypothetical protein	-
C9888_RS06560	1243710..1244081	+	372	WP_014417525.1	hypothetical protein	-
C9888_RS06565	1244086..1244283	+	198	WP_007610833.1	XkdX family protein	-
C9888_RS06570	1244340..1245101	+	762	WP_014417526.1	hypothetical protein	-
C9888_RS06575	1245153..1245416	+	264	WP_003154815.1	hemolysin XhlA family protein	-
C9888_RS06580	1245430..1245693	+	264	WP_003154813.1	phage holin	-
C9888_RS06585	1245707..1246585	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
C9888_RS06590	1246842..1247012	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
C9888_RS06595	1247013..1247759	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
C9888_RS06600	1247863..1248861	-	999	WP_003154805.1	inorganic phosphate transporter	-
C9888_RS06605	1248874..1249491	-	618	WP_003154804.1	DUF47 domain-containing protein	-
C9888_RS06610	1249777..1251093	-	1317	WP_007610842.1	amino acid permease	-
C9888_RS06615	1251415..1252365	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1232989..1255975	22986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T101049 WP_007407256.1 NZ_CP028375:c1247759-1247013 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T101049 NZ_CP028375:c1247759-1247013 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT101049 WP_014417527.1 NZ_CP028375:c1247012-1246842 [Bacillus velezensis]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT101049 NZ_CP028375:c1247012-1246842 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8