Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 468374..469011 Replicon chromosome
Accession NZ_CP028375
Organism Bacillus velezensis strain W1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C9888_RS02425 Protein ID WP_003156187.1
Coordinates 468661..469011 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag C9888_RS02420 Protein ID WP_003156188.1
Coordinates 468374..468655 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9888_RS02400 464739..465338 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
C9888_RS02405 465431..465796 + 366 WP_014304402.1 holo-ACP synthase -
C9888_RS02410 465961..466968 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
C9888_RS02415 467085..468254 + 1170 WP_014416966.1 alanine racemase -
C9888_RS02420 468374..468655 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C9888_RS02425 468661..469011 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C9888_RS02430 469129..469950 + 822 WP_003156182.1 STAS domain-containing protein -
C9888_RS02435 469955..470320 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
C9888_RS02440 470323..470724 + 402 WP_003156178.1 anti-sigma regulatory factor -
C9888_RS02445 470736..471743 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
C9888_RS02450 471807..472136 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
C9888_RS02455 472133..472615 + 483 WP_007609591.1 anti-sigma B factor RsbW -
C9888_RS02460 472581..473369 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
C9888_RS02465 473369..473971 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T101048 WP_003156187.1 NZ_CP028375:468661-469011 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T101048 NZ_CP028375:468661-469011 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT101048 WP_003156188.1 NZ_CP028375:468374-468655 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT101048 NZ_CP028375:468374-468655 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References