Detailed information of TA system TA101042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3393903..3395650
• Replicon: chromosome
• Accession: NZ_CP028367
• Organism: Halomonas sp. SF2003

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: C8233_RS14165
• Protein ID: WP_107336030.1
• Coordinates: 3394997..3395650 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: C8233_RS14160
• Protein ID: WP_107336029.1
• Coordinates: 3393903..3394997 (-)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8233_RS14150	3389151..3392516	-	3366	WP_107336027.1	type I restriction endonuclease subunit R	-
C8233_RS14155	3392596..3393477	-	882	WP_107336028.1	hypothetical protein	-
C8233_RS14160	3393903..3394997	-	1095	WP_107336029.1	macro domain-containing protein	Antitoxin
C8233_RS14165	3394997..3395650	-	654	WP_107336030.1	DUF4433 domain-containing protein	Toxin
C8233_RS14170	3395654..3397477	-	1824	WP_107336031.1	DUF262 domain-containing protein	-
C8233_RS14175	3397517..3398722	-	1206	WP_107336032.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3385066..3404344	19278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 24798.40 Da
• Isoelectric Point: 6.9127

>T101042 WP_107336030.1 NZ_CP028367:c3395650-3394997 [Halomonas sp. SF2003]
MAIDLSRSLNPQKGLIFRITHRNNLPWILANGLHCRSAEHQDRGFITIGRQELIDKRATREVPVVPGGVLSDYIPFYFTP
SSMMLYNIVTGHGVARQNREDIIVLVSDLPTLMQQGIGFLFTNQHAYLASAEFSDRLEDLTEYIPWGLLQAKDFKRDLEQ
PDKTDRYQAEALVHRHLPISALRGIGTYTDEVCRDVETLVAAHQLPLKVVTRPNWFF

Nucleotide

• Length: 654 bp

>T101042 NZ_CP028367:c3395650-3394997 [Halomonas sp. SF2003]
ATGGCCATTGATCTCTCGCGCAGCCTGAATCCACAAAAGGGGCTGATCTTTCGGATCACCCATCGCAACAATCTGCCATG
GATTCTGGCCAACGGCCTTCACTGCCGTTCAGCAGAACACCAGGACCGGGGGTTCATCACCATCGGACGGCAGGAGCTGA
TCGACAAACGGGCAACGCGAGAGGTTCCGGTAGTGCCAGGCGGCGTATTGAGCGACTACATTCCCTTCTATTTTACGCCT
AGCTCCATGATGCTATACAACATCGTCACGGGGCATGGCGTGGCCAGGCAGAATAGGGAAGATATCATCGTGCTGGTCAG
CGACCTGCCAACACTCATGCAGCAAGGGATTGGCTTTCTATTCACCAATCAGCATGCCTACCTGGCCAGCGCCGAGTTCA
GTGATCGCCTGGAAGACTTGACCGAGTACATCCCTTGGGGACTCCTTCAGGCCAAGGACTTCAAGCGTGACCTTGAACAA
CCAGACAAGACTGATCGATATCAAGCCGAAGCACTCGTCCATCGCCATCTGCCCATCTCGGCATTGCGGGGTATTGGCAC
GTATACTGACGAAGTGTGCCGTGATGTCGAAACACTCGTCGCAGCGCACCAGCTCCCCCTGAAAGTGGTGACACGACCCA
ACTGGTTCTTCTGA

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 41192.20 Da
• Isoelectric Point: 5.3433

>AT101042 WP_107336029.1 NZ_CP028367:c3394997-3393903 [Halomonas sp. SF2003]
MIEYTRGNLLEADVEALINTVNTVGVMGKGIALMFKEAFPDNFAAYAAACKHREIETGRMFVTERDALLGPRWIINFPTK
QHWRARTQMEWIESGLDDLKRVIRERGIRSIALPPLGCGNGGLDWEDVRPRIEAALSELEDVHVLVFEPTNQYQNVAKRS
GVETLTPARALLAELVRRYWVLGNECSLLEVQKLAWLLERRILAHDLDNPLKLDFKPHRYGPYSDRLRHLLNGLDGSYLH
SEKRINDATPDEVVWFDESRRALINAYLRSAEAAPYAEVLEEVDALIDGFQSPLGMEVLATLDWLITQESVTPSVEAIKE
GLRHWPNDLAGQRKLKLFSDRLIALALERLISGPRPPIALGAES

Nucleotide

• Length: 1095 bp

>AT101042 NZ_CP028367:c3394997-3393903 [Halomonas sp. SF2003]
ATGATTGAGTACACCCGTGGCAACCTCCTGGAAGCCGATGTCGAGGCTTTGATCAATACCGTCAACACCGTGGGTGTGAT
GGGGAAAGGCATCGCCTTGATGTTCAAGGAGGCCTTTCCAGACAACTTTGCCGCCTATGCCGCTGCCTGCAAGCATCGCG
AGATCGAAACCGGCCGGATGTTCGTCACCGAGCGCGATGCTCTCCTGGGTCCACGCTGGATCATCAACTTCCCGACCAAG
CAGCATTGGCGTGCCAGAACGCAGATGGAATGGATCGAATCCGGCCTGGATGACCTCAAGCGGGTAATCCGCGAGCGGGG
TATACGCTCCATTGCCCTGCCGCCACTGGGCTGCGGCAATGGCGGGCTCGACTGGGAGGATGTTCGGCCGCGTATCGAGG
CAGCCCTGAGTGAATTGGAGGATGTTCACGTACTGGTCTTCGAGCCGACCAACCAATATCAGAATGTGGCCAAGCGTAGT
GGCGTAGAGACGCTGACGCCAGCGCGTGCCCTGCTGGCTGAACTGGTGCGTCGCTACTGGGTACTGGGAAACGAGTGCTC
ACTGCTGGAGGTGCAAAAGCTGGCGTGGCTACTGGAGCGCCGCATTCTTGCCCACGATCTCGATAATCCTCTCAAGCTCG
ACTTCAAGCCGCACCGCTACGGCCCCTACTCGGATCGTCTTCGCCATCTGCTCAATGGCCTCGACGGCAGCTATCTGCAT
AGCGAGAAGCGCATCAATGATGCCACTCCCGATGAGGTGGTGTGGTTCGATGAATCGCGCCGAGCACTCATCAATGCCTA
CCTGCGCAGCGCCGAGGCAGCCCCCTATGCCGAGGTGCTGGAAGAAGTCGATGCCCTGATCGATGGCTTCCAGTCGCCAC
TCGGGATGGAGGTGCTGGCAACTCTTGACTGGTTGATCACTCAGGAAAGCGTCACACCTAGCGTAGAAGCCATCAAGGAA
GGGCTCAGGCACTGGCCTAATGACCTGGCCGGGCAGCGCAAGCTGAAGCTGTTCTCGGACCGCCTGATCGCTCTGGCACT
GGAGAGGCTGATATCAGGCCCAAGGCCGCCCATCGCCTTGGGCGCGGAGTCATGA