Detailed information of TA system TA101026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 362592..363193
• Replicon: plasmid pPV989-508
• Accession: NZ_CP028350
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: C9381_RS20820
• Protein ID: WP_107320392.1
• Coordinates: 362592..362975 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6YQJ8
• Locus tag: C9381_RS20825
• Protein ID: WP_013196184.1
• Coordinates: 362972..363193 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS20810	357952..360354	+	2403	WP_107320390.1	maltodextrin phosphorylase	-
C9381_RS20815	360363..362429	+	2067	WP_107320391.1	4-alpha-glucanotransferase	-
C9381_RS20820	362592..362975	-	384	WP_107320392.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C9381_RS20825	362972..363193	-	222	WP_013196184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C9381_RS20830	363388..364323	-	936	WP_107320393.1	maltose operon protein MalM	-
C9381_RS20835	364422..365723	-	1302	WP_013196182.1	maltoporin	-
C9381_RS20840	365767..366876	-	1110	WP_013196181.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN / pilW	1..507680	507680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (5-115)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-58)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14225.45 Da
• Isoelectric Point: 5.7250

>T101026 WP_107320392.1 NZ_CP028350:c362975-362592 [Pantoea vagans]
MKWVSAQEVIAFHDRLLAVLPGIKGMPEPQRAEAIIYRVQNQLYYEGVKDIFVLAAIYLIAISRGHIFNDGNKRTAFFVT
MVFLNRNGIAIRDEDNELEELTVSAATGDATTEVIAAVLRRLSFLSP

Nucleotide

• Length: 384 bp

>T101026 NZ_CP028350:c362975-362592 [Pantoea vagans]
ATGAAATGGGTTAGTGCTCAGGAGGTGATAGCTTTTCATGATCGTCTGCTGGCTGTTCTGCCAGGTATCAAGGGTATGCC
AGAACCCCAGAGAGCAGAAGCTATCATTTATAGGGTACAGAACCAGCTCTATTATGAAGGTGTGAAAGATATCTTTGTAT
TAGCCGCCATCTACCTCATCGCTATATCCCGGGGACACATTTTTAATGATGGTAATAAGCGAACTGCATTTTTCGTCACG
ATGGTTTTTTTGAATCGTAACGGCATAGCTATACGTGATGAAGATAATGAACTCGAAGAATTAACGGTATCTGCTGCTAC
TGGTGATGCTACGACTGAGGTCATTGCTGCTGTATTGCGGCGATTATCATTTTTGTCTCCGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8145.02 Da
• Isoelectric Point: 4.7527

>AT101026 WP_013196184.1 NZ_CP028350:c363193-362972 [Pantoea vagans]
MRSYTTTEARQNIAEVMDTATAGEPVEITRRDGSSAVLISRDDFNAWQEAKLDAEFADIMGRHSRTVKALADR

Nucleotide

• Length: 222 bp

>AT101026 NZ_CP028350:c363193-362972 [Pantoea vagans]
ATGCGTAGTTACACCACCACTGAGGCCCGACAAAACATTGCTGAAGTCATGGACACAGCTACGGCCGGTGAGCCTGTAGA
AATAACCCGGCGCGATGGTTCTTCTGCTGTACTTATCAGTCGCGACGATTTTAATGCCTGGCAGGAAGCAAAACTGGATG
CCGAATTTGCCGACATTATGGGGCGACATTCACGTACGGTGAAGGCACTGGCTGACCGATGA

Structures

Antitoxin

• AlphaFold DB: D6YQJ8