Detailed information of TA system TA101023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4007351..4007898
• Replicon: chromosome
• Accession: NZ_CP028349
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C9381_RS18790
• Protein ID: WP_033784085.1
• Coordinates: 4007351..4007659 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E1SEY8
• Locus tag: C9381_RS18795
• Protein ID: WP_013359125.1
• Coordinates: 4007659..4007898 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS18775	4003332..4004318	+	987	WP_107320125.1	Gfo/Idh/MocA family oxidoreductase	-
C9381_RS18780	4004574..4005542	+	969	WP_013359123.1	TerC family protein	-
C9381_RS18785	4005778..4007010	+	1233	WP_033784084.1	serine/threonine transporter SstT	-
C9381_RS18790	4007351..4007659	-	309	WP_033784085.1	CcdB family protein	Toxin
C9381_RS18795	4007659..4007898	-	240	WP_013359125.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C9381_RS18800	4008177..4009667	-	1491	WP_033784086.1	altronate dehydratase family protein	-
C9381_RS18805	4009672..4011120	-	1449	WP_033784087.1	tagaturonate reductase	-
C9381_RS18810	4011123..4012538	-	1416	WP_033784088.1	glucuronate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (10-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11706.63 Da
• Isoelectric Point: 7.2013

>T101023 WP_033784085.1 NZ_CP028349:c4007659-4007351 [Pantoea vagans]
MQFDVYRNTGKAEIYPLVLDVQSDIIGRRKTRMAIPLFPHENYKGPRADRLTPILVIEGQKYVMMTHEMASVPEKALRED
VENISGQAVVIKSALDFLFDRI

Nucleotide

• Length: 309 bp

>T101023 NZ_CP028349:c4007659-4007351 [Pantoea vagans]
ATGCAGTTTGACGTTTACAGGAACACCGGGAAGGCGGAGATATACCCATTAGTTTTGGACGTTCAGAGCGACATCATTGG
CCGGCGGAAAACCCGGATGGCTATTCCTCTGTTCCCGCATGAGAACTACAAAGGGCCACGTGCTGACCGGCTAACGCCAA
TTCTGGTCATAGAGGGGCAAAAATACGTGATGATGACCCACGAAATGGCGAGTGTACCGGAAAAGGCGCTACGCGAAGAC
GTGGAGAACATCAGCGGTCAGGCGGTGGTGATAAAATCTGCCCTCGATTTTCTGTTTGATCGTATCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8840.89 Da
• Isoelectric Point: 5.6032

>AT101023 WP_013359125.1 NZ_CP028349:c4007898-4007659 [Pantoea vagans]
MGEVATKPTKNINVTIDTKLALEAREAGINFSQTLAKAVRAELKSLAAERWKHENADAIDYLNQLSDEHGLFSDEHRTF

Nucleotide

• Length: 240 bp

>AT101023 NZ_CP028349:c4007898-4007659 [Pantoea vagans]
ATGGGTGAAGTAGCCACAAAACCAACGAAAAACATCAACGTCACTATCGACACGAAACTGGCCCTGGAAGCGCGTGAAGC
AGGGATTAACTTCTCTCAGACACTTGCTAAAGCGGTGCGCGCAGAGCTGAAATCTCTCGCAGCAGAACGCTGGAAACATG
AAAACGCGGACGCTATCGACTATCTGAACCAGCTCAGTGATGAGCATGGCCTTTTCTCTGACGAACACAGGACGTTCTGA

Structures

Antitoxin

• AlphaFold DB: E1SEY8