Detailed information of TA system TA101022

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3643132..3643870
• Replicon: chromosome
• Accession: NZ_CP028349
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C9381_RS17100
• Protein ID: WP_107320049.1
• Coordinates: 3643490..3643870 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C9381_RS17095
• Protein ID: WP_107320048.1
• Coordinates: 3643132..3643458 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS17065	3639320..3640189	+	870	WP_107320042.1	GTPase family protein	-
C9381_RS17070	3640450..3641145	+	696	WP_107320043.1	transcriptional regulator	-
C9381_RS17075	3641166..3641639	+	474	WP_107320044.1	hypothetical protein	-
C9381_RS17080	3641664..3642122	+	459	WP_107320045.1	hypothetical protein	-
C9381_RS17085	3642220..3642639	+	420	WP_107320046.1	antirestriction protein	-
C9381_RS17090	3642654..3643121	+	468	WP_107320047.1	DNA repair protein RadC	-
C9381_RS17095	3643132..3643458	+	327	WP_107320048.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C9381_RS17100	3643490..3643870	+	381	WP_107320049.1	TA system toxin CbtA family protein	Toxin
C9381_RS17105	3643867..3644076	+	210	WP_107320050.1	hypothetical protein	-
C9381_RS17110	3644611..3645332	+	722	Protein_3305	hypothetical protein	-
C9381_RS17115	3645335..3645808	+	474	WP_107320051.1	hypothetical protein	-
C9381_RS17120	3646290..3647111	+	822	WP_107320052.1	hypothetical protein	-
C9381_RS17125	3647119..3647415	+	297	WP_095706782.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3618869..3647111	28242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14072.92 Da
• Isoelectric Point: 6.2173

>T101022 WP_107320049.1 NZ_CP028349:3643490-3643870 [Pantoea vagans]
MQNLPLPEPRAAEPCPSPEQVWQMLLTHLLDKHYGLTLNDTPFGDDSVIQAHINAGISLCDAVNFIVERYELVRTDNSRF
NLSERSSLIGAIDILRARRATGLIVSSGYSTITSITTGRYRENKTQ

Nucleotide

• Length: 381 bp

>T101022 NZ_CP028349:3643490-3643870 [Pantoea vagans]
ATGCAAAATTTACCCTTACCCGAACCACGGGCGGCTGAACCCTGCCCGTCGCCCGAGCAGGTCTGGCAAATGCTGCTCAC
ACATCTGCTGGATAAACACTATGGCCTCACGCTCAACGACACACCGTTTGGCGACGACAGCGTGATTCAGGCGCACATCA
ACGCCGGTATCTCCCTGTGTGATGCTGTCAATTTCATTGTGGAAAGGTATGAACTGGTGCGCACCGACAACAGCCGTTTC
AACCTCAGCGAGCGCTCATCGCTGATTGGTGCCATCGATATTCTGCGTGCCCGTCGTGCAACCGGACTGATAGTAAGCAG
CGGCTACAGTACCATAACCAGCATCACAACGGGCCGGTATCGAGAGAACAAAACACAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12067.75 Da
• Isoelectric Point: 7.0373

>AT101022 WP_107320048.1 NZ_CP028349:3643132-3643458 [Pantoea vagans]
MHSERWGLTCDKTPCFGARLVQEGHRLHFLADRAGFNGDFSNTEALRLDQAFPLIMKQLEQMLSTGELNPRLQQRVTLHH
IGLTCQADTLGSHGYVYIAIYPSPAVTG

Nucleotide

• Length: 327 bp

>AT101022 NZ_CP028349:3643132-3643458 [Pantoea vagans]
ATGCATTCTGAACGATGGGGGCTCACGTGCGATAAAACGCCGTGTTTCGGTGCCAGGCTGGTGCAGGAAGGCCACCGGCT
GCACTTTCTGGCTGACCGGGCAGGATTTAACGGCGACTTCAGCAACACTGAAGCACTGCGTCTGGACCAGGCTTTTCCGC
TAATAATGAAACAGCTGGAACAGATGCTTTCAACTGGAGAGCTGAATCCCCGCCTTCAGCAACGCGTTACCCTGCATCAC
ATTGGGCTGACCTGCCAAGCCGATACGCTCGGTTCACACGGTTACGTTTACATCGCGATTTATCCCTCTCCGGCAGTCAC
CGGCTGA