Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | slvAT/- |
| Location | 78680..79596 | Replicon | plasmid pTTS12 |
| Accession | NZ_CP009975 | ||
| Organism | Pseudomonas putida S12 | ||
Toxin (Protein)
| Gene name | slvT | Uniprot ID | A0A2L1KI32 |
| Locus tag | RPPX_RS26260 | Protein ID | WP_019437783.1 |
| Coordinates | 79072..79596 (+) | Length | 175 a.a. |
Antitoxin (Protein)
| Gene name | slvA | Uniprot ID | A0A2L1KH13 |
| Locus tag | RPPX_RS26255 | Protein ID | WP_019437782.1 |
| Coordinates | 78680..79075 (+) | Length | 132 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RPPX_RS26235 (RPPX_26235) | 74340..74864 | - | 525 | WP_010794447.1 | hypothetical protein | - |
| RPPX_RS26245 (RPPX_26245) | 75731..76012 | - | 282 | WP_019437780.1 | hypothetical protein | - |
| RPPX_RS26250 (RPPX_26250) | 76176..78095 | + | 1920 | WP_026031886.1 | hypothetical protein | - |
| RPPX_RS26255 (RPPX_26255) | 78680..79075 | + | 396 | WP_019437782.1 | hypothetical protein | Antitoxin |
| RPPX_RS26260 (RPPX_26260) | 79072..79596 | + | 525 | WP_019437783.1 | RES family NAD+ phosphorylase | Toxin |
| RPPX_RS26265 (RPPX_26265) | 79642..80382 | - | 741 | Protein_74 | stress response protein TerZ | - |
| RPPX_RS26270 (RPPX_26270) | 80588..82102 | + | 1515 | WP_019437638.1 | IS21-like element ISPpu7 family transposase | - |
| RPPX_RS26275 (RPPX_26275) | 82120..82875 | + | 756 | WP_019437637.1 | IS21-like element ISPpu7 family helper ATPase IstB | - |
| RPPX_RS26280 (RPPX_26280) | 82980..83495 | - | 516 | Protein_77 | TerD family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..583900 | 583900 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 175 a.a. Molecular weight: 19052.57 Da Isoelectric Point: 4.5535
>T10102 WP_019437783.1 NZ_CP009975:79072-79596 [Pseudomonas putida S12]
MKIIGVQDLPCYRMHTPKWSVAPTSGAGAATQGGRVNRKGLEALYLSLESATAIAEFQQASAFLPPGLLISYSLSLDRVI
DFTGGYNDTWAPIWQDFFCDWRKIRFNDGVEPPSWIIGDLAIEADCAGILFESVANPGGRNLVLFTDQLPVHGNIVVNDP
RGDLPTDQSSWTRP
MKIIGVQDLPCYRMHTPKWSVAPTSGAGAATQGGRVNRKGLEALYLSLESATAIAEFQQASAFLPPGLLISYSLSLDRVI
DFTGGYNDTWAPIWQDFFCDWRKIRFNDGVEPPSWIIGDLAIEADCAGILFESVANPGGRNLVLFTDQLPVHGNIVVNDP
RGDLPTDQSSWTRP
Download Length: 525 bp
>T10102 NZ_CP009975:79072-79596 [Pseudomonas putida S12]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCTCCTGGTCTGCTGATCAGCTATTCCCTCAGTCTGGATCGAGTGATC
GACTTCACCGGCGGCTATAACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTATCGGGGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCTCCTGGTCTGCTGATCAGCTATTCCCTCAGTCTGGATCGAGTGATC
GACTTCACCGGCGGCTATAACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTATCGGGGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG
Antitoxin
Download Length: 132 a.a. Molecular weight: 14753.87 Da Isoelectric Point: 7.2048
>AT10102 WP_019437782.1 NZ_CP009975:78680-79075 [Pseudomonas putida S12]
MASKAVLELRPTNFAGLMDALKEVNRVMFSPERFLKVFSMDQQTLALRAHVHRNTVRNAPESEKVQAYIRDSVKVLRAVT
DMGTDVTNAIFWFKNEPLSTFGYKTAEEVVSEGKTEQLIAFLQSWEAGAQG
MASKAVLELRPTNFAGLMDALKEVNRVMFSPERFLKVFSMDQQTLALRAHVHRNTVRNAPESEKVQAYIRDSVKVLRAVT
DMGTDVTNAIFWFKNEPLSTFGYKTAEEVVSEGKTEQLIAFLQSWEAGAQG
Download Length: 396 bp
>AT10102 NZ_CP009975:78680-79075 [Pseudomonas putida S12]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGCGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCGGCTATAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCTTGGGAAGCTGGTGCCCAAGGATGA
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGCGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCGGCTATAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCTTGGGAAGCTGGTGCCCAAGGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2L1KI32 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2L1KH13 |
References
(1) Hadiastri Kusumawardhani et al. (2020) Novel Toxin-Antitoxin Module SlvT-SlvA Regulates Megaplasmid Stability and Incites Solvent Tolerance in Pseudomonas putida S12. Applied And Environmental Microbiology 86(13):e00686-20. [PubMed:32358012]