Detailed information of TA system TA101018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2463246..2463878
• Replicon: chromosome
• Accession: NZ_CP028349
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5M7LEN8
• Locus tag: C9381_RS11700
• Protein ID: WP_052271421.1
• Coordinates: 2463246..2463422 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C9381_RS11705
• Protein ID: WP_107319672.1
• Coordinates: 2463471..2463878 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS11660	2458803..2459759	+	957	WP_199852946.1	DUF550 domain-containing protein	-
C9381_RS11665	2459756..2460052	+	297	WP_107319666.1	hypothetical protein	-
C9381_RS11670	2460049..2460246	+	198	WP_107319667.1	hypothetical protein	-
C9381_RS11675	2460243..2460608	+	366	WP_107320177.1	RusA family crossover junction endodeoxyribonuclease	-
C9381_RS11680	2460763..2461479	+	717	WP_107319668.1	phage antirepressor KilAC domain-containing protein	-
C9381_RS11685	2461476..2462492	+	1017	WP_107319669.1	DUF968 domain-containing protein	-
C9381_RS11690	2462514..2462942	+	429	WP_107319670.1	antitermination protein Q	-
C9381_RS11700	2463246..2463422	+	177	WP_052271421.1	type II toxin-antitoxin system HicA family toxin	Toxin
C9381_RS11705	2463471..2463878	+	408	WP_107319672.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C9381_RS11710	2464035..2464388	+	354	WP_107319673.1	phage holin, lambda family	-
C9381_RS11715	2464372..2464797	+	426	WP_107319674.1	lysozyme	-
C9381_RS11720	2464794..2465228	+	435	WP_107319675.1	Rz lytic protein	-
C9381_RS11730	2465382..2465621	+	240	WP_107319677.1	hypothetical protein	-
C9381_RS11735	2465664..2465852	-	189	WP_107319678.1	hypothetical protein	-
C9381_RS11745	2466304..2467761	+	1458	WP_107319680.1	glycosyltransferase	-
C9381_RS11750	2467743..2468360	+	618	WP_199852947.1	hypothetical protein	-
C9381_RS11755	2468329..2468682	+	354	WP_107319682.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2449532..2488691	39159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-63)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6727.86 Da
• Isoelectric Point: 11.4032

>T101018 WP_052271421.1 NZ_CP028349:2463246-2463422 [Pantoea vagans]
VKQSEFRRWLESQGVEVSNGTNHLKLRYNGKRSVMPRHPGAELKEPLRKAIMKQLGLK

Nucleotide

• Length: 177 bp

>T101018 NZ_CP028349:2463246-2463422 [Pantoea vagans]
GTGAAGCAAAGCGAGTTCAGGCGGTGGCTTGAATCTCAGGGAGTCGAAGTTTCAAACGGTACTAACCATCTGAAGCTGAG
ATACAACGGGAAGCGAAGTGTAATGCCGAGGCATCCCGGTGCTGAGTTAAAAGAACCACTGCGAAAGGCCATAATGAAGC
AGTTAGGCCTGAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14636.73 Da
• Isoelectric Point: 4.3596

>AT101018 WP_107319672.1 NZ_CP028349:2463471-2463878 [Pantoea vagans]
MRYPINLEPCDGGYVVSFPDIPEALTQGDTREEALEMGLDALVTSFDFYFEDNQPVPAPGPVTGDFVEVPASVSAKVLLL
NAFLASGLTQVELASRMGVKKQEVTRIFDLHHSTKIDTVQKALSALGKRLELVAA

Nucleotide

• Length: 408 bp

>AT101018 NZ_CP028349:2463471-2463878 [Pantoea vagans]
ATGCGATACCCGATTAATCTTGAGCCGTGCGACGGCGGATATGTGGTTTCGTTCCCGGATATACCGGAGGCGCTTACTCA
GGGCGATACGCGTGAGGAGGCGTTAGAGATGGGGCTGGATGCGCTGGTTACTTCATTTGATTTCTACTTTGAAGATAACC
AGCCTGTTCCGGCACCTGGACCGGTGACAGGGGATTTTGTAGAGGTTCCGGCGAGTGTGTCGGCGAAGGTGCTATTGCTA
AATGCTTTCCTTGCTTCCGGCTTAACTCAGGTTGAGCTGGCTTCACGCATGGGAGTTAAAAAGCAGGAGGTAACGCGCAT
CTTCGATCTGCATCACTCGACCAAAATAGATACTGTTCAGAAGGCGCTCTCAGCGCTGGGCAAGCGGCTTGAATTAGTCG
CTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A5M7LEN8