Detailed information of TA system TA101017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2430766..2431396
• Replicon: chromosome
• Accession: NZ_CP028349
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A379AGD8
• Locus tag: C9381_RS11500
• Protein ID: WP_010245321.1
• Coordinates: 2430766..2430948 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C9381_RS11505
• Protein ID: WP_033783026.1
• Coordinates: 2430971..2431396 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS11480	2426114..2427511	+	1398	WP_107319645.1	class II fumarate hydratase	-
C9381_RS11485	2427553..2428482	-	930	WP_033783022.1	DNA replication terminus site-binding protein	-
C9381_RS11490	2428669..2429868	+	1200	WP_033783024.1	MFS transporter	-
C9381_RS11495	2430076..2430570	+	495	WP_013357792.1	Rrf2 family transcriptional regulator	-
C9381_RS11500	2430766..2430948	+	183	WP_010245321.1	type II toxin-antitoxin system HicA family toxin	Toxin
C9381_RS11505	2430971..2431396	+	426	WP_033783026.1	hypothetical protein	Antitoxin
C9381_RS11510	2431479..2431784	+	306	WP_095707239.1	type II toxin-antitoxin system HicB family antitoxin	-
C9381_RS11515	2431853..2435005	-	3153	WP_033783027.1	multidrug efflux RND transporter permease subunit OqxB	-
C9381_RS11520	2435018..2436172	-	1155	WP_013357795.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7000.12 Da
• Isoelectric Point: 11.0643

>T101017 WP_010245321.1 NZ_CP028349:2430766-2430948 [Pantoea vagans]
MKYSEFRRWLIQQGAILTKHRSGSSHFKVFLGDRQSIFPDHGAKEMPEGTRRKILKDLGL

Nucleotide

• Length: 183 bp

>T101017 NZ_CP028349:2430766-2430948 [Pantoea vagans]
ATGAAGTACAGTGAATTCAGACGCTGGCTCATTCAGCAGGGCGCTATTTTGACTAAGCACCGCAGTGGAAGCAGCCATTT
CAAGGTCTTCCTGGGCGACAGGCAATCCATTTTTCCGGATCATGGAGCGAAAGAAATGCCTGAAGGTACGCGCAGAAAAA
TTTTAAAAGATCTGGGCCTGTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15405.77 Da
• Isoelectric Point: 5.2470

>AT101017 WP_033783026.1 NZ_CP028349:2430971-2431396 [Pantoea vagans]
MFNYAVSLTHDAKTDTYEVSCRDLPLLHSVGATVEEALSEAKDGLVTAISLEMDTRRPIPVASKPLEGEYIVSVPVLVAM
KAALYNAMIETGTRKVDLARQLGVNGPQVDRLLDVEHSSKVEKVEMALAQLGRKAQIEIRT

Nucleotide

• Length: 426 bp

>AT101017 NZ_CP028349:2430971-2431396 [Pantoea vagans]
ATGTTTAATTATGCCGTCAGCTTAACCCATGACGCCAAAACCGATACCTATGAGGTCTCGTGTCGTGATTTGCCGTTACT
GCATTCCGTCGGCGCCACCGTTGAGGAAGCACTGTCAGAAGCCAAAGATGGACTGGTGACGGCTATCTCTCTTGAGATGG
ATACCCGTCGTCCGATTCCGGTCGCCAGTAAACCGCTGGAAGGTGAATACATTGTCTCCGTACCTGTGCTGGTGGCGATG
AAAGCGGCACTTTATAACGCAATGATTGAAACCGGCACTCGTAAAGTCGATCTGGCACGCCAGCTGGGCGTGAATGGCCC
ACAGGTTGATCGACTGCTGGATGTTGAGCACTCATCGAAAGTTGAAAAAGTGGAGATGGCGCTGGCGCAACTGGGACGAA
AAGCCCAAATTGAGATTCGCACCTGA

Structures

Toxin

• AlphaFold DB: A0A379AGD8