Detailed information of TA system TA101016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2264441..2265025
• Replicon: chromosome
• Accession: NZ_CP028349
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C9381_RS10675
• Protein ID: WP_033782894.1
• Coordinates: 2264441..2264776 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C9381_RS10680
• Protein ID: WP_033782896.1
• Coordinates: 2264777..2265025 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS10665	2260064..2262682	+	2619	WP_107319605.1	nitrate reductase	-
C9381_RS10670	2262682..2263563	+	882	WP_051926489.1	uroporphyrinogen-III C-methyltransferase	-
C9381_RS10675	2264441..2264776	-	336	WP_033782894.1	endoribonuclease MazF	Toxin
C9381_RS10680	2264777..2265025	-	249	WP_033782896.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C9381_RS10685	2265174..2265986	-	813	WP_033782898.1	DeoR/GlpR family DNA-binding transcription regulator	-
C9381_RS10690	2266339..2267136	+	798	WP_033782900.1	D-threitol dehydrogenase	-
C9381_RS10695	2267192..2268127	+	936	WP_033732417.1	D-ribose ABC transporter substrate-binding protein	-
C9381_RS10700	2268173..2268784	+	612	WP_033732419.1	DUF2291 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• Antitoxin PemI-like (3-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 11999.00 Da
• Isoelectric Point: 9.2256

>T101016 WP_033782894.1 NZ_CP028349:c2264776-2264441 [Pantoea vagans]
MVSRFVPEAGDIIWVDFDPIKGSEQAGHRPAVVLSPFAYNNKTGMCVCVPCTTHSKGYPFEVPISAEKDSVALSDQVKSI
AWKVRGAIKKGVVSASELQQIRGKIKVIIGL

Nucleotide

• Length: 336 bp

>T101016 NZ_CP028349:c2264776-2264441 [Pantoea vagans]
ATGGTAAGCCGTTTTGTGCCTGAAGCCGGAGATATCATCTGGGTGGATTTTGATCCGATCAAAGGAAGTGAGCAGGCAGG
TCACAGGCCAGCTGTTGTTCTTAGCCCGTTTGCTTACAACAATAAAACCGGCATGTGTGTCTGTGTACCCTGCACAACGC
ATTCAAAGGGATATCCGTTCGAAGTTCCCATAAGCGCTGAGAAAGATAGCGTTGCCCTATCCGACCAGGTAAAAAGCATC
GCCTGGAAAGTCAGGGGGGCCATCAAAAAAGGTGTCGTTTCCGCAAGCGAACTTCAGCAAATTCGTGGAAAGATAAAAGT
CATCATCGGTTTATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9213.58 Da
• Isoelectric Point: 4.2459

>AT101016 WP_033782896.1 NZ_CP028349:c2265025-2264777 [Pantoea vagans]
MIHGNVKRWGNSPAIRIPATLMQALNLNIDDEIKIDLVEGKLIIEPVKQEVGYSLAELLEGVTEENLHDPVEWGEPVGKE
IW

Nucleotide

• Length: 249 bp

>AT101016 NZ_CP028349:c2265025-2264777 [Pantoea vagans]
ATGATTCATGGAAATGTTAAACGCTGGGGTAACTCTCCAGCAATACGTATCCCGGCGACATTGATGCAGGCACTCAATCT
TAATATTGATGACGAAATAAAAATCGATTTGGTTGAAGGAAAACTCATTATTGAACCTGTAAAGCAGGAAGTTGGTTACT
CTCTTGCAGAACTGCTTGAAGGCGTGACAGAAGAAAATCTGCACGATCCGGTTGAATGGGGTGAGCCTGTCGGGAAGGAG
ATTTGGTAA