101015

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1494185..1494802 Replicon chromosome
Accession NZ_CP028349
Organism Pantoea vagans strain PV989

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag C9381_RS06930 Protein ID WP_003850458.1
Coordinates 1494185..1494400 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E1SGC9
Locus tag C9381_RS06935 Protein ID WP_013357010.1
Coordinates 1494425..1494802 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9381_RS06900 1490087..1490425 + 339 WP_003850469.1 P-II family nitrogen regulator -
C9381_RS06905 1490460..1491746 + 1287 WP_013357006.1 ammonium transporter AmtB -
C9381_RS06910 1491805..1492668 - 864 WP_013357007.1 acyl-CoA thioesterase II -
C9381_RS06915 1492859..1493416 + 558 WP_013357008.1 YbaY family lipoprotein -
C9381_RS06920 1493418..1493762 - 345 WP_013357009.1 MGMT family protein -
C9381_RS06930 1494185..1494400 - 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
C9381_RS06935 1494425..1494802 - 378 WP_013357010.1 Hha toxicity modulator TomB Antitoxin
C9381_RS06940 1494950..1495303 - 354 WP_041456682.1 hypothetical protein -
C9381_RS06945 1495633..1496301 + 669 WP_033782191.1 ABC transporter ATP-binding protein -
C9381_RS06950 1496298..1497137 + 840 WP_107319385.1 metal ABC transporter permease -
C9381_RS06955 1497157..1498035 + 879 WP_107319386.1 metal ABC transporter substrate-binding protein -
C9381_RS06960 1498081..1498221 - 141 WP_009091664.1 type B 50S ribosomal protein L36 -
C9381_RS06965 1498233..1498487 - 255 WP_033782196.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T101015 WP_003850458.1 NZ_CP028349:c1494400-1494185 [Pantoea vagans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T101015 NZ_CP028349:c1494400-1494185 [Pantoea vagans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGTTGCCGCTCTCTGGATACCCTGGAAAGGGTCAT
TGAAAAAAATAAGTATGAATTACCCGAGGATGAATTAGCGGTATTTTACTCTGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTTTATGATAAAGTCCCAGGTTCAGTATGGAAATTTGTCCGCTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14650.28 Da        Isoelectric Point: 4.3976

>AT101015 WP_013357010.1 NZ_CP028349:c1494802-1494425 [Pantoea vagans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGINSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT101015 NZ_CP028349:c1494802-1494425 [Pantoea vagans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATATCTGTGTGAGAGCCTGTTTGACGACAGCATGGC
AACATTAACCGACAGCCATCACGGCTGGGTGAACGACCCCACTTCTGAAAGCAATCTGCAGCTTAACGATTTGATTGAAC
ACATTGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAACTATGGTATCAATAGTCCGGATCTTCAACGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCAAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A5M7PH97

References