Detailed information of TA system TA101012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 430603..431171
• Replicon: plasmid pTHN
• Accession: NZ_CP028348
• Organism: Novosphingobium sp. THN1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C7W88_RS19095
• Protein ID: WP_118075136.1
• Coordinates: 430839..431171 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C7W88_RS19090
• Protein ID: WP_118075890.1
• Coordinates: 430603..430845 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7W88_RS19070	426573..427499	+	927	WP_118075132.1	polysaccharide deacetylase	-
C7W88_RS19075	427496..428647	+	1152	WP_118075133.1	FAD-dependent monooxygenase	-
C7W88_RS19080	428644..430152	+	1509	WP_118075134.1	aldehyde dehydrogenase family protein	-
C7W88_RS19085	430201..430542	+	342	WP_118075135.1	hypothetical protein	-
C7W88_RS19090	430603..430845	+	243	WP_118075890.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C7W88_RS19095	430839..431171	+	333	WP_118075136.1	endoribonuclease MazF	Toxin
C7W88_RS19100	431194..431985	-	792	WP_118075137.1	helix-turn-helix domain-containing protein	-
C7W88_RS19105	432105..433226	-	1122	WP_118075892.1	alcohol dehydrogenase catalytic domain-containing protein	-
C7W88_RS19110	433226..434086	-	861	WP_118075138.1	isocitrate lyase/PEP mutase family protein	-
C7W88_RS19115	434200..436059	-	1860	WP_118075139.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1161559	1161559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• SpoVT-AbrB domain (6-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12081.23 Da
• Isoelectric Point: 10.1426

>T101012 WP_118075136.1 NZ_CP028348:430839-431171 [Novosphingobium sp. THN1]
MVSAYVPAAGDVVWLQFDPQAGHEQAGHRPALVLSPQRYNKARGMMLCCPMTSRIKGYPFEVVVSVEPPSAVLADQIKAL
DWRARKATHKGKVPEAVLAEVRAKLKALIF

Nucleotide

• Length: 333 bp

>T101012 NZ_CP028348:430839-431171 [Novosphingobium sp. THN1]
CTGGTGAGTGCCTACGTCCCGGCGGCGGGCGACGTTGTTTGGCTGCAGTTCGATCCGCAGGCCGGGCACGAACAGGCAGG
CCATCGTCCCGCCCTGGTGCTGTCGCCGCAGCGCTACAACAAGGCGCGGGGCATGATGCTGTGCTGCCCGATGACCTCGC
GGATCAAAGGCTATCCCTTTGAAGTCGTGGTCAGCGTCGAGCCGCCGAGCGCCGTTCTGGCCGATCAGATCAAGGCGCTC
GATTGGCGAGCGCGCAAGGCGACGCACAAGGGCAAGGTGCCAGAAGCGGTCCTTGCCGAGGTGCGCGCCAAGCTAAAGGC
GCTGATCTTCTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8548.73 Da
• Isoelectric Point: 4.1225

>AT101012 WP_118075890.1 NZ_CP028348:430603-430845 [Novosphingobium sp. THN1]
MQVQVKKWGNSASVRIPASVLAAASLKIDQAVDVREEGGRVIIEPIAEPRFDLDELLAAMTPESFPEDVAFGPAVGGEAW

Nucleotide

• Length: 243 bp

>AT101012 NZ_CP028348:430603-430845 [Novosphingobium sp. THN1]
ATGCAGGTGCAGGTAAAGAAGTGGGGCAACAGCGCTTCGGTGCGCATCCCCGCATCGGTGCTCGCTGCGGCCTCGCTGAA
GATCGATCAGGCGGTCGACGTGCGTGAGGAGGGCGGCCGGGTGATTATCGAGCCGATTGCCGAACCGCGCTTCGATCTCG
ATGAACTGCTCGCCGCGATGACGCCCGAGAGTTTTCCCGAAGACGTCGCGTTCGGCCCTGCCGTTGGCGGTGAAGCCTGG
TGA