Detailed information of TA system TA101009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /FR47-DUF1778
• Location: 1120..1906
• Replicon: plasmid pVpR13_19Kb
• Accession: NZ_CP028344
• Organism: Vibrio parahaemolyticus isolate R13

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C7Y67_RS25865
• Protein ID: WP_108745467.1
• Coordinates: 1394..1906 (+)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C7Y67_RS25860
• Protein ID: WP_108745466.1
• Coordinates: 1120..1413 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7Y67_RS25855	99..476	-	378	WP_108745465.1	hypothetical protein	-
C7Y67_RS25860	1120..1413	+	294	WP_108745466.1	DUF1778 domain-containing protein	Antitoxin
C7Y67_RS25865	1394..1906	+	513	WP_108745467.1	GNAT family N-acetyltransferase	Toxin
C7Y67_RS25870	2074..2316	+	243	WP_108745469.1	hypothetical protein	-
C7Y67_RS25875	2479..2895	-	417	WP_108745468.1	hypothetical protein	-
C7Y67_RS25880	3104..6106	-	3003	WP_031419875.1	Tn3 family transposase	-
C7Y67_RS25885	6264..6821	+	558	WP_031419876.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19217	19217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• FR47-like (86-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-88)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 18724.37 Da
• Isoelectric Point: 6.6347

>T101009 WP_108745467.1 NZ_CP028344:1394-1906 [Vibrio parahaemolyticus]
MNIEHLDKNTHDRNAFDCGEPALNTYLKATSGQHNKKDLSRTFVLTSSGNPSQIKGFYSLALCTVELDELPENIAKKYPS
AIHCALIGRLAVNKSHQRQGLGSVLLIDAVRKAIESSKAIPTPMIIVDAKNDIAKSMYIDLGFESFPNQDNRLFMTTATA
IAMLNEIDSE

Nucleotide

• Length: 513 bp

>T101009 NZ_CP028344:1394-1906 [Vibrio parahaemolyticus]
ATGAATATAGAGCATCTTGATAAAAATACACATGATAGAAACGCATTCGACTGTGGCGAACCTGCGTTAAACACCTACCT
AAAAGCAACGTCAGGCCAACATAATAAAAAAGACCTGTCTCGTACCTTTGTTTTAACAAGCAGTGGTAACCCCTCTCAAA
TTAAAGGTTTTTACTCTCTGGCTTTATGCACTGTAGAGTTAGATGAACTACCTGAAAACATAGCTAAAAAGTATCCCTCT
GCAATACATTGCGCTTTAATTGGTCGATTGGCTGTTAACAAATCTCACCAACGTCAAGGATTAGGGTCTGTCCTCCTTAT
TGATGCTGTTCGAAAGGCTATAGAATCATCGAAAGCTATACCGACTCCAATGATAATCGTTGATGCAAAAAACGATATCG
CTAAAAGCATGTATATTGATTTAGGTTTTGAGTCGTTCCCTAATCAAGACAATCGATTATTCATGACCACGGCTACAGCA
ATAGCCATGTTGAATGAAATTGACTCAGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11009.62 Da
• Isoelectric Point: 10.0967

>AT101009 WP_108745466.1 NZ_CP028344:1120-1413 [Vibrio parahaemolyticus]
MATLTNERITTRVNSETKELLERALSLSGYASLNSFIANAAVAEAKRLIEQNMRIKLCQEDALAFVNALERPVKTNDRFL
RAARKHKETIINEYRAS

Nucleotide

• Length: 294 bp

>AT101009 NZ_CP028344:1120-1413 [Vibrio parahaemolyticus]
ATGGCCACATTAACTAATGAAAGAATCACCACTAGGGTGAATTCAGAAACCAAAGAGTTGCTCGAAAGAGCGTTGTCATT
GTCAGGGTACGCTAGTTTGAATAGTTTTATTGCGAATGCTGCTGTTGCGGAAGCTAAGAGACTTATTGAACAAAACATGC
GTATTAAGCTGTGTCAGGAGGATGCTTTAGCTTTTGTTAATGCATTAGAGCGTCCAGTTAAGACAAATGACCGTTTCCTT
CGAGCTGCTCGTAAGCATAAGGAAACAATAATAAATGAATATAGAGCATCTTGA