Detailed information of TA system TA101008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 1305768..1306284
• Replicon: chromosome
• Accession: NZ_CP028342
• Organism: Vibrio parahaemolyticus isolate R13

Toxin (Protein)

• Gene name: relB
• Uniprot ID: U2ZZV8
• Locus tag: C7Y67_RS06785
• Protein ID: WP_005464459.1
• Coordinates: 1306015..1306284 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: C7Y67_RS06780
• Protein ID: WP_025548943.1
• Coordinates: 1305768..1306022 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7Y67_RS06745	1301756..1302244	+	489	WP_025549022.1	hypothetical protein	-
C7Y67_RS06750	1302381..1302797	+	417	WP_025507116.1	hypothetical protein	-
C7Y67_RS26715	1302763..1302912	+	150	WP_162457646.1	DUF3265 domain-containing protein	-
C7Y67_RS06760	1303009..1303605	+	597	WP_078532239.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
C7Y67_RS06770	1304835..1304990	+	156	Protein_1176	SDR family oxidoreductase	-
C7Y67_RS06775	1305278..1305544	+	267	WP_005464625.1	hypothetical protein	-
C7Y67_RS06780	1305768..1306022	+	255	WP_025548943.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C7Y67_RS06785	1306015..1306284	+	270	WP_005464459.1	Txe/YoeB family addiction module toxin	Toxin
C7Y67_RS06790	1306501..1306722	+	222	Protein_1180	transposase	-
C7Y67_RS06795	1306806..1307606	+	801	WP_086482363.1	IS3 family transposase	-
C7Y67_RS26720	1307975..1308030	+	56	Protein_1182	DUF3265 domain-containing protein	-
C7Y67_RS06805	1308217..1309107	+	891	WP_005485669.1	hypothetical protein	-
C7Y67_RS06810	1309188..1309598	+	411	WP_024701711.1	hypothetical protein	-
C7Y67_RS06815	1309777..1310210	+	434	Protein_1185	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1244387..1313644	69257
	-	inside	Integron	-	-	1244911..1309743	64832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10614.96 Da
• Isoelectric Point: 7.9816

>T101008 WP_005464459.1 NZ_CP028342:1306015-1306284 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T101008 NZ_CP028342:1306015-1306284 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9511.68 Da
• Isoelectric Point: 5.0623

>AT101008 WP_025548943.1 NZ_CP028342:1305768-1306022 [Vibrio parahaemolyticus]
MRIVSFTEARNSLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT101008 NZ_CP028342:1305768-1306022 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATAGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: U2ZZV8