Detailed information of TA system TA101007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-DinJ
• Location: 1346281..1346832
• Replicon: chromosome
• Accession: NZ_CP028341
• Organism: Bifidobacterium adolescentis strain 1-11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C8077_RS06050
• Protein ID: WP_199850063.1
• Coordinates: 1346281..1346532 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A087DRE9
• Locus tag: C8077_RS06055
• Protein ID: WP_033494219.1
• Coordinates: 1346587..1346832 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8077_RS06025	1341353..1342030	-	678	WP_107646316.1	DUF1819 family protein	-
C8077_RS06030	1342374..1343612	-	1239	WP_198051532.1	GTP-binding DUF697 domain-containing protein	-
C8077_RS06035	1344007..1345470	+	1464	WP_107646317.1	hypothetical protein	-
C8077_RS06040	1345531..1345884	-	354	WP_107646630.1	DUF86 domain-containing protein	-
C8077_RS06045	1345931..1346284	-	354	WP_011743341.1	nucleotidyltransferase domain-containing protein	-
C8077_RS06050	1346281..1346532	-	252	WP_199850063.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C8077_RS06055	1346587..1346832	-	246	WP_033494219.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C8077_RS06060	1347060..1347872	+	813	WP_085381954.1	metallophosphoesterase	-
C8077_RS06065	1347890..1348570	-	681	WP_107646318.1	ECF transporter S component	-
C8077_RS06070	1348862..1349614	+	753	WP_107646319.1	YdcF family protein	-
C8077_RS06075	1349678..1350310	-	633	WP_107646320.1	3'-5' exonuclease	-
C8077_RS06080	1350446..1351810	-	1365	WP_003810410.1	trigger factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1336235..1346832	10597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-80)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-52)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9174.65 Da
• Isoelectric Point: 4.9889

>T101007 WP_199850063.1 NZ_CP028341:c1346532-1346281 [Bifidobacterium adolescentis]
MQNDEIEGFDSTVVCLMTSFTSDDMTTRVKVPPTAQNGLIKISWGMTEKILAVHTSALGQRIGILEDDVMRQVEKKLAHV
LAL

Nucleotide

• Length: 252 bp

>T101007 NZ_CP028341:c1346532-1346281 [Bifidobacterium adolescentis]
TTGCAGAATGACGAAATCGAGGGTTTTGACTCTACCGTGGTATGCCTGATGACTTCTTTTACGTCGGATGATATGACTAC
CCGGGTTAAAGTTCCTCCTACTGCGCAGAATGGTTTGATTAAAATAAGTTGGGGGATGACGGAGAAGATTCTTGCAGTGC
ACACTTCGGCATTGGGCCAACGCATTGGAATTTTGGAAGATGATGTCATGCGCCAGGTGGAAAAGAAACTTGCACATGTT
CTAGCTCTATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9328.31 Da
• Isoelectric Point: 4.0948

>AT101007 WP_033494219.1 NZ_CP028341:c1346832-1346587 [Bifidobacterium adolescentis]
MVVQLATRVDDDQAEEFRETTKKLGLSTADALRMFVSSFNEYQGFPYQVRVRQVAEPFENEREASDFANDLSMRMIADEE
G

Nucleotide

• Length: 246 bp

>AT101007 NZ_CP028341:c1346832-1346587 [Bifidobacterium adolescentis]
ATGGTAGTTCAGCTTGCGACACGTGTTGATGATGATCAGGCGGAAGAGTTCCGTGAAACGACAAAGAAGCTCGGTCTTTC
CACGGCTGATGCACTGCGTATGTTCGTCTCGTCCTTTAATGAATATCAGGGGTTCCCATACCAGGTACGAGTTCGACAGG
TTGCGGAACCGTTCGAGAATGAACGGGAGGCTTCTGATTTCGCTAATGATTTGTCGATGAGGATGATCGCTGATGAGGAG
GGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A087DRE9