Detailed information of TA system TA100998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2071759..2072537
• Replicon: chromosome
• Accession: NZ_CP028339
• Organism: Thauera aromatica K172

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: Tharo_RS09800
• Protein ID: WP_211309589.1
• Coordinates: 2072031..2072537 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: Tharo_RS09795
• Protein ID: WP_107221019.1
• Coordinates: 2071759..2072046 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tharo_RS09775	2067313..2067576	-	264	WP_107221016.1	hypothetical protein	-
Tharo_RS09780	2067848..2069140	+	1293	WP_107221017.1	serine--tRNA ligase	-
Tharo_RS09785	2069668..2071050	-	1383	WP_107221018.1	membrane-bound lytic murein transglycosylase MltF	-
Tharo_RS09790	2071117..2071575	-	459	WP_211309683.1	cupin domain-containing protein	-
Tharo_RS09795	2071759..2072046	+	288	WP_107221019.1	DUF1778 domain-containing protein	Antitoxin
Tharo_RS09800	2072031..2072537	+	507	WP_211309589.1	GNAT family N-acetyltransferase	Toxin
Tharo_RS17735	2072558..2072722	-	165	WP_211309590.1	WecB/TagA/CpsF family glycosyltransferase	-
Tharo_RS09810	2072686..2073009	-	324	WP_107221021.1	DUF86 domain-containing protein	-
Tharo_RS09815	2073340..2073741	-	402	WP_107221022.1	PIN domain-containing protein	-
Tharo_RS09820	2073741..2073974	-	234	WP_107221023.1	antitoxin	-
Tharo_RS17740	2074204..2074470	+	267	WP_211309591.1	hypothetical protein	-
Tharo_RS09830	2074694..2075659	-	966	WP_107221024.1	magnesium/cobalt transporter CorA	-
Tharo_RS09835	2075686..2077455	-	1770	WP_107221025.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (47-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-87)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 17838.54 Da
• Isoelectric Point: 6.4769

>T100998 WP_211309589.1 NZ_CP028339:2072031-2072537 [Thauera aromatica K172]
MGSGVTLAAPEPLAAHHDTDAFSCGVESLDRWLKQRALKNQATGASRTFVVCEDNRVLAYYALASSAVAAELATGRLRRT
MPDPIPVLVLGRLAIDQSLRGQGIGRALVRDACLRVMAAADAIGIRGLLVHALSPEAQAFYEHVGFESSPLEPMTLMATI
ADVRAGLE

Nucleotide

• Length: 507 bp

>T100998 NZ_CP028339:2072031-2072537 [Thauera aromatica K172]
ATGGGATCGGGTGTGACGCTGGCGGCGCCCGAGCCTCTGGCGGCACATCACGACACCGACGCATTCTCCTGCGGCGTCGA
GAGTCTGGATCGCTGGCTCAAGCAACGGGCCTTGAAGAACCAGGCAACGGGTGCTTCACGTACATTCGTGGTATGCGAGG
ACAACCGGGTGCTGGCCTACTACGCTCTGGCCTCCAGTGCTGTCGCTGCCGAGCTGGCCACAGGGCGTTTGCGGCGCACT
ATGCCTGATCCGATTCCTGTGCTTGTGCTCGGTCGCCTGGCCATCGACCAGTCCTTGCGTGGCCAGGGCATCGGCCGCGC
CCTGGTACGTGATGCGTGCTTGCGGGTGATGGCGGCAGCCGACGCCATTGGCATTCGGGGGCTGCTGGTGCATGCGCTCT
CACCTGAAGCGCAGGCATTTTACGAGCATGTCGGCTTTGAGTCCTCGCCGCTGGAGCCCATGACCTTGATGGCCACTATC
GCGGATGTGCGTGCAGGGCTGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10685.35 Da
• Isoelectric Point: 10.9149

>AT100998 WP_107221019.1 NZ_CP028339:2071759-2072046 [Thauera aromatica K172]
MPTVEPVAKRDTLNLRIKPEVRGLIDRAAKVRGKNRTDFILDAARQAAEETLLDQALIRVSPQAYAEFVARLDRPVAPNA
RLQKTLQSPAPWDRV

Nucleotide

• Length: 288 bp

>AT100998 NZ_CP028339:2071759-2072046 [Thauera aromatica K172]
ATGCCCACGGTCGAACCCGTCGCCAAGCGCGACACACTCAACCTGCGCATCAAACCTGAGGTGCGCGGTCTGATTGACCG
TGCTGCCAAGGTGCGCGGCAAGAATCGCACGGACTTCATCCTCGATGCCGCTCGTCAGGCGGCAGAAGAGACCTTGCTCG
ACCAGGCGCTGATCCGCGTCAGCCCCCAAGCCTATGCCGAGTTTGTGGCGCGCCTGGATCGCCCGGTAGCGCCCAACGCC
CGTCTGCAAAAGACACTTCAATCGCCTGCCCCATGGGATCGGGTGTGA