Detailed information of TA system TA100983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapD-relB/-
• Location: 647465..648122
• Replicon: chromosome
• Accession: NZ_CP028325
• Organism: Helicobacter pylori strain FDAARGOS_298

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: K2KCG4
• Locus tag: CEP77_RS03190
• Protein ID: WP_000271048.1
• Coordinates: 647838..648122 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CEP77_RS03185
• Protein ID: WP_108169271.1
• Coordinates: 647465..647857 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP77_RS03165	643202..643957	+	756	WP_108169268.1	HugZ family heme oxygenase	-
CEP77_RS03175	644254..646488	+	2235	WP_108169269.1	Hop family adhesin BabA	-
CEP77_RS03185	647465..647857	+	393	WP_108169271.1	hypothetical protein	Antitoxin
CEP77_RS03190	647838..648122	+	285	WP_000271048.1	endoribonuclease VapD	Toxin
CEP77_RS03195	648628..649773	+	1146	WP_108169272.1	YbfB/YjiJ family MFS transporter	-
CEP77_RS03200	649779..650741	-	963	WP_108169273.1	GTP-binding protein	-
CEP77_RS03205	650741..651109	-	369	WP_108169274.1	hypothetical protein	-
CEP77_RS03210	651204..652322	+	1119	WP_108169275.1	site-specific DNA-methyltransferase	-
CEP77_RS03215	652324..653016	+	693	WP_108169276.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11144.69 Da
• Isoelectric Point: 4.8896

>T100983 WP_000271048.1 NZ_CP028325:647838-648122 [Helicobacter pylori]
MYALAFDLKIEILKKEYGEPYNKAYDDLRQELELLGFENTQGSVYVNYSKENTLVQVYKAINKLSQIEWFKKSVRDIRAF
KVEDFSDFTEIVKS

Nucleotide

• Length: 285 bp

>T100983 NZ_CP028325:647838-648122 [Helicobacter pylori]
ATGTATGCTTTAGCGTTTGATTTAAAGATTGAGATTTTAAAAAAAGAATACGGAGAACCCTACAATAAAGCCTATGATGA
TTTAAGGCAAGAATTAGAGCTATTAGGGTTTGAAAACACTCAAGGGAGCGTTTATGTTAATTATTCTAAAGAAAACACTT
TAGTGCAAGTCTATAAAGCGATCAATAAACTCTCTCAAATTGAGTGGTTTAAAAAGTCTGTTAGGGATATTAGAGCGTTT
AAGGTAGAGGACTTTAGCGATTTTACTGAGATTGTGAAATCCTAG

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15282.29 Da
• Isoelectric Point: 6.4703

>AT100983 WP_108169271.1 NZ_CP028325:647465-647857 [Helicobacter pylori]
MPNTTAEKDYTKYSEKQLFNLIHQLERKIKKMQNDRVSFKEKMAKELEKRDQNFKDKIDALNELLQKISQAFDDKRDCCL
GHEIPNIQTQQAMRDAVNGVNLTQIDNLDDLTNELKRENNKGFENVCFSV

Nucleotide

• Length: 393 bp

>AT100983 NZ_CP028325:647465-647857 [Helicobacter pylori]
ATGCCAAACACCACCGCAGAAAAAGACTACACAAAATACAGCGAAAAACAGCTTTTTAATTTAATCCATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAGTTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCATGAGATCCCAAACATTCAAACGCAACAAGCCATGAGAGATGCGGTGAATGGGGTTAATCTCACTCAAATTGATAA
TTTAGATGATCTTACAAACGAGTTAAAAAGAGAAAATAATAAAGGTTTTGAAAATGTATGCTTTAGCGTTTGA

Structures

Toxin

• AlphaFold DB: A0A2X5A8S4