Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9231..9756 Replicon plasmid p0.1229_1
Accession NZ_CP028321
Organism Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C7X15_RS24775 Protein ID WP_001159871.1
Coordinates 9451..9756 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C7X15_RS24770 Protein ID WP_000813634.1
Coordinates 9231..9449 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7X15_RS24760 6268..6909 - 642 WP_000990667.1 hypothetical protein -
C7X15_RS24770 9231..9449 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C7X15_RS24775 9451..9756 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C7X15_RS24780 9757..10566 + 810 WP_000016979.1 site-specific integrase -
C7X15_RS24785 10704..10979 - 276 WP_000239529.1 hypothetical protein -
C7X15_RS24790 10973..11617 - 645 WP_000633911.1 AAA family ATPase -
C7X15_RS24795 11846..12817 + 972 WP_001103694.1 hypothetical protein -
C7X15_RS24800 12822..13214 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
C7X15_RS24805 13219..14489 - 1271 Protein_14 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114229 114229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T100982 WP_001159871.1 NZ_CP028321:9451-9756 [Escherichia coli O18:H1]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T100982 NZ_CP028321:9451-9756 [Escherichia coli O18:H1]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT100982 WP_000813634.1 NZ_CP028321:9231-9449 [Escherichia coli O18:H1]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT100982 NZ_CP028321:9231-9449 [Escherichia coli O18:H1]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References