Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1491803..1492421 Replicon chromosome
Accession NZ_CP028320
Organism Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C7X15_RS07225 Protein ID WP_001291435.1
Coordinates 1491803..1492021 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C7X15_RS07230 Protein ID WP_000344800.1
Coordinates 1492047..1492421 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7X15_RS07190 1487091..1487663 + 573 WP_000779831.1 YbaY family lipoprotein -
C7X15_RS07195 1487694..1488005 - 312 WP_000409908.1 MGMT family protein -
C7X15_RS07205 1488384..1488737 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C7X15_RS07210 1488779..1490329 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C7X15_RS07215 1490493..1490963 - 471 WP_001304825.1 YlaC family protein -
C7X15_RS07220 1491079..1491630 - 552 WP_000102539.1 maltose O-acetyltransferase -
C7X15_RS07225 1491803..1492021 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C7X15_RS07230 1492047..1492421 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C7X15_RS07235 1492966..1496115 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C7X15_RS07240 1496138..1497331 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100964 WP_001291435.1 NZ_CP028320:c1492021-1491803 [Escherichia coli O18:H1]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T100964 NZ_CP028320:c1492021-1491803 [Escherichia coli O18:H1]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT100964 WP_000344800.1 NZ_CP028320:c1492421-1492047 [Escherichia coli O18:H1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT100964 NZ_CP028320:c1492421-1492047 [Escherichia coli O18:H1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References