Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 999076..999626 Replicon chromosome
Accession NZ_CP028320
Organism Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag C7X15_RS04855 Protein ID WP_001160966.1
Coordinates 999312..999626 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C7X15_RS04850 Protein ID WP_000125566.1
Coordinates 999076..999309 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7X15_RS04825 994149..994436 + 288 WP_000203741.1 ferredoxin-like protein FixX -
C7X15_RS04830 994495..995826 + 1332 WP_001183207.1 MFS transporter -
C7X15_RS04835 995934..996464 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C7X15_RS04840 996457..998319 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
C7X15_RS04845 998511..998990 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
C7X15_RS04850 999076..999309 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C7X15_RS04855 999312..999626 + 315 WP_001160966.1 CcdB family protein Toxin
C7X15_RS04860 999623..1000471 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C7X15_RS04865 1000478..1000855 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C7X15_RS04870 1000858..1001679 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C7X15_RS04875 1001676..1002665 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C7X15_RS04880 1002665..1003951 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T100962 WP_001160966.1 NZ_CP028320:999312-999626 [Escherichia coli O18:H1]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T100962 NZ_CP028320:999312-999626 [Escherichia coli O18:H1]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT100962 WP_000125566.1 NZ_CP028320:999076-999309 [Escherichia coli O18:H1]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT100962 NZ_CP028320:999076-999309 [Escherichia coli O18:H1]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References