Detailed information of TA system TA100955

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 829450..830285
• Replicon: chromosome
• Accession: NZ_CP028320
• Organism: Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2S4A272
• Locus tag: C7X15_RS04045
• Protein ID: WP_001094443.1
• Coordinates: 829908..830285 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: C7X15_RS04040
• Protein ID: WP_001285607.1
• Coordinates: 829450..829818 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7X15_RS04005	825342..826022	+	681	WP_001282919.1	WYL domain-containing protein	-
C7X15_RS04010	826170..826847	+	678	WP_001097301.1	hypothetical protein	-
C7X15_RS04015	826853..827086	+	234	WP_001278287.1	DUF905 family protein	-
C7X15_RS04020	827176..827994	+	819	WP_059335041.1	DUF945 domain-containing protein	-
C7X15_RS04025	828086..828571	+	486	WP_000206664.1	antirestriction protein	-
C7X15_RS04030	828586..829062	+	477	WP_001186165.1	RadC family protein	-
C7X15_RS04035	829149..829370	+	222	WP_000692350.1	DUF987 domain-containing protein	-
C7X15_RS04040	829450..829818	+	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C7X15_RS04045	829908..830285	+	378	WP_001094443.1	TA system toxin CbtA family protein	Toxin
C7X15_RS04050	830282..830770	+	489	WP_000761685.1	hypothetical protein	-
C7X15_RS04055	830790..830987	+	198	WP_001327226.1	DUF957 domain-containing protein	-
C7X15_RS26335	831072..831218	+	147	WP_001290178.1	hypothetical protein	-
C7X15_RS04065	831682..832755	+	1074	WP_001327207.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.97 Da
• Isoelectric Point: 7.3523

>T100955 WP_001094443.1 NZ_CP028320:829908-830285 [Escherichia coli O18:H1]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T100955 NZ_CP028320:829908-830285 [Escherichia coli O18:H1]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT100955 WP_001285607.1 NZ_CP028320:829450-829818 [Escherichia coli O18:H1]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT100955 NZ_CP028320:829450-829818 [Escherichia coli O18:H1]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2S4A272

Antitoxin

• AlphaFold DB: A0A1M0JLU8