Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 646210..646707 Replicon chromosome
Accession NZ_CP028320
Organism Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C7X15_RS03110 Protein ID WP_001173343.1
Coordinates 646534..646707 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag C7X15_RS03105 Protein ID WP_001312331.1
Coordinates 646210..646506 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7X15_RS03085 642939..643658 - 720 WP_000611291.1 two-component system response regulator DcuR -
C7X15_RS03090 643655..645286 - 1632 WP_001216466.1 sensor histidine kinase -
C7X15_RS03095 645467..645697 + 231 WP_000371715.1 (4Fe-4S)-binding protein -
C7X15_RS03100 645709..645981 + 273 WP_000405647.1 N-acetyltransferase -
C7X15_RS03105 646210..646506 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C7X15_RS03110 646534..646707 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C7X15_RS03115 646826..648343 - 1518 WP_000003806.1 lysine--tRNA ligase -
C7X15_RS03120 648580..650037 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T100954 WP_001173343.1 NZ_CP028320:646534-646707 [Escherichia coli O18:H1]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T100954 NZ_CP028320:646534-646707 [Escherichia coli O18:H1]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT100954 WP_001312331.1 NZ_CP028320:646210-646506 [Escherichia coli O18:H1]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT100954 NZ_CP028320:646210-646506 [Escherichia coli O18:H1]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References