Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 503994..504576 Replicon chromosome
Accession NZ_CP028285
Organism Enterococcus faecalis strain FDAARGOS_324

Toxin (Protein)


Gene name zeta Uniprot ID A0A0M2AE74
Locus tag CEQ02_RS03220 Protein ID WP_002355414.1
Coordinates 504268..504576 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag CEQ02_RS03215 Protein ID WP_002326825.1
Coordinates 503994..504266 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ02_RS03190 499631..499897 + 267 WP_002384819.1 hypothetical protein -
CEQ02_RS03195 499887..501351 + 1465 Protein_452 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
CEQ02_RS03205 502837..503691 + 855 WP_002364921.1 ParA family protein -
CEQ02_RS03210 503762..503977 + 216 WP_002364919.1 peptide-binding protein -
CEQ02_RS03215 503994..504266 + 273 WP_002326825.1 antitoxin Antitoxin
CEQ02_RS03220 504268..504576 + 309 WP_002355414.1 zeta toxin family protein Toxin
CEQ02_RS03225 504656..505084 - 429 WP_071623404.1 tyrosine-type recombinase/integrase -
CEQ02_RS03230 505129..505629 - 501 WP_002355415.1 HAD family hydrolase -
CEQ02_RS03235 505634..506401 - 768 WP_002384821.1 DeoR/GlpR family DNA-binding transcription regulator -
CEQ02_RS03245 506889..507314 + 426 WP_002355418.1 galactose-6-phosphate isomerase subunit LacA -
CEQ02_RS03250 507331..507846 + 516 WP_002345825.1 galactose-6-phosphate isomerase subunit LacB -
CEQ02_RS03255 507857..508789 + 933 WP_002363040.1 tagatose-6-phosphate kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-99)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11525.91 Da        Isoelectric Point: 5.7251

>T100941 WP_002355414.1 NZ_CP028285:504268-504576 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Download         Length: 309 bp

>T100941 NZ_CP028285:504268-504576 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT100941 WP_002326825.1 NZ_CP028285:503994-504266 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT100941 NZ_CP028285:503994-504266 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AE74

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References