Detailed information of TA system TA100940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 149743..150314
• Replicon: chromosome
• Accession: NZ_CP028285
• Organism: Enterococcus faecalis strain FDAARGOS_324

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CEQ02_RS01470
• Protein ID: WP_002385138.1
• Coordinates: 149973..150314 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: CEQ02_RS01465
• Protein ID: WP_002354773.1
• Coordinates: 149743..149973 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ02_RS01440	144785..146401	-	1617	WP_107164636.1	phosphatase PAP2/LCP family protein	-
CEQ02_RS01450	147089..147727	+	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
CEQ02_RS01455	147991..148983	-	993	WP_002385136.1	biotin--[acetyl-CoA-carboxylase] ligase	-
CEQ02_RS01460	149122..149337	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
CEQ02_RS01465	149743..149973	+	231	WP_002354773.1	hypothetical protein	Antitoxin
CEQ02_RS01470	149973..150314	+	342	WP_002385138.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEQ02_RS01480	150685..154299	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13128.35 Da
• Isoelectric Point: 9.3984

>T100940 WP_002385138.1 NZ_CP028285:149973-150314 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAAICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T100940 NZ_CP028285:149973-150314 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGCGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT100940 WP_002354773.1 NZ_CP028285:149743-149973 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT100940 NZ_CP028285:149743-149973 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812