Detailed information of TA system TA100929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 77720..78399
• Replicon: plasmid unnamed1
• Accession: NZ_CP028272
• Organism: Mixta intestinalis strain SRCM103226

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C7M51_RS22155
• Protein ID: WP_160623811.1
• Coordinates: 77947..78399 (+)
• Length: 151 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: C7M51_RS22150
• Protein ID: WP_160623810.1
• Coordinates: 77720..77950 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M51_RS22125	73559..74227	-	669	WP_160623805.1	hypothetical protein	-
C7M51_RS22130	74283..75056	-	774	WP_160623806.1	site-specific integrase	-
C7M51_RS22135	75331..75876	-	546	WP_160623807.1	hypothetical protein	-
C7M51_RS22140	75873..76076	-	204	WP_167522398.1	hypothetical protein	-
C7M51_RS22145	76177..77100	-	924	WP_160623809.1	hypothetical protein	-
C7M51_RS22150	77720..77950	+	231	WP_160623810.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C7M51_RS22155	77947..78399	+	453	WP_160623811.1	type II toxin-antitoxin system VapC family toxin	Toxin
C7M51_RS22160	78423..78701	+	279	WP_160623812.1	hypothetical protein	-
C7M51_RS22165	78771..79115	+	345	WP_160250113.1	hypothetical protein	-
C7M51_RS22170	79133..80404	-	1272	WP_160623813.1	hypothetical protein	-
C7M51_RS22175	80958..81284	-	327	WP_160623814.1	hypothetical protein	-
C7M51_RS22180	81315..81647	-	333	WP_160623815.1	hypothetical protein	-
C7M51_RS22185	82166..83161	+	996	WP_160623816.1	nucleotidyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85320	85320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 151 a.a.
• Molecular weight: 16266.84 Da
• Isoelectric Point: 8.2930

>T100929 WP_160623811.1 NZ_CP028272:77947-78399 [Mixta intestinalis]
VNSIYMLDTNICSFIMREQPEAVLTRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHMQLVDAFCSRLDAILPWDRA
AVDATAEIKVALRLAGTPIGPNNTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWANVREAGMRRPACS

Nucleotide

• Length: 453 bp

>T100929 NZ_CP028272:77947-78399 [Mixta intestinalis]
GTGAACAGTATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGACGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACTTACTCCGAGATGCGCTTCGGCGCTACCGGCC
CGAAGGCCTCGCCCCGCCACATGCAGCTGGTTGACGCGTTCTGCTCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCGACCGCGGAGATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGCCCGAACAACACGGCGATTGC
CGGGCATGCCATTGCGGCAGGGGCGATTCTGGTGACGAACAATGTCAGGGAGTTTGCGCGGGTGCCGGGGTTAGTACTTG
AAGACTGGGCGAACGTCCGGGAGGCTGGTATGAGGCGCCCCGCCTGTTCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8691.82 Da
• Isoelectric Point: 4.6017

>AT100929 WP_160623810.1 NZ_CP028272:77720-77950 [Mixta intestinalis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQMEKANPDFMAEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT100929 NZ_CP028272:77720-77950 [Mixta intestinalis]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGCTTACCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGATGG
AAAAAGCAAACCCGGACTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA