Detailed information of TA system TA100926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 23356..23957
• Replicon: plasmid unnamed3
• Accession: NZ_CP028244
• Organism: Lactiplantibacillus plantarum strain SRCM101518

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: C7M40_RS16030
• Protein ID: WP_001748110.1
• Coordinates: 23356..23700 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: C7M40_RS16035
• Protein ID: WP_001748109.1
• Coordinates: 23694..23957 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M40_RS16010	19282..20697	+	1416	WP_119463189.1	glycoside hydrolase family 32 protein	-
C7M40_RS16015	21025..22572	-	1548	WP_160231011.1	IS66 family transposase	-
C7M40_RS16020	22644..22991	-	348	WP_160231010.1	IS66 family insertion sequence element accessory protein TnpB	-
C7M40_RS16025	22981..23163	-	183	WP_057874333.1	hypothetical protein	-
C7M40_RS16030	23356..23700	-	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C7M40_RS16035	23694..23957	-	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C7M40_RS16040	24043..24630	+	588	WP_160231363.1	site-specific integrase	-
C7M40_RS16045	24858..25085	+	228	WP_173819272.1	hypothetical protein	-
C7M40_RS16050	25108..25386	+	279	WP_160231365.1	hypothetical protein	-
C7M40_RS16055	25376..25516	-	141	Protein_31	ATPase	-
C7M40_RS16060	25537..25722	-	186	WP_010620892.1	hypothetical protein	-
C7M40_RS16065	25769..26050	-	282	WP_003660204.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C7M40_RS16070	26277..27353	-	1077	WP_160231366.1	replication initiator protein A	-
C7M40_RS16075	28016..28219	-	204	WP_015474751.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38506	38506

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T100926 WP_001748110.1 NZ_CP028244:c23700-23356 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T100926 NZ_CP028244:c23700-23356 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCGAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT100926 WP_001748109.1 NZ_CP028244:c23957-23694 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT100926 NZ_CP028244:c23957-23694 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A806J926