Detailed information of TA system TA100925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 7611..8212
• Replicon: plasmid unnamed5
• Accession: NZ_CP028234
• Organism: Lactiplantibacillus plantarum strain SRCM101222

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: C7M38_RS15960
• Protein ID: WP_001748110.1
• Coordinates: 7868..8212 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: C7M38_RS15955
• Protein ID: WP_160248642.1
• Coordinates: 7611..7874 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M38_RS15925	3222..3689	+	468	WP_003587210.1	DNA starvation/stationary phase protection protein	-
C7M38_RS15930	3731..3820	-	90	Protein_7	IS30 family transposase	-
C7M38_RS15935	4046..4549	+	504	WP_027822201.1	DUF536 domain-containing protein	-
C7M38_RS15940	5262..5639	+	378	WP_160248638.1	YxeA family protein	-
C7M38_RS15945	6368..6580	-	213	WP_010015136.1	hypothetical protein	-
C7M38_RS15950	6936..7523	-	588	WP_160248640.1	site-specific integrase	-
C7M38_RS15955	7611..7874	+	264	WP_160248642.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C7M38_RS15960	7868..8212	+	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C7M38_RS15965	8466..8747	-	282	WP_080241820.1	IS3 family transposase	-
C7M38_RS15970	9105..10274	+	1170	WP_160248645.1	SH3 domain-containing protein	-
C7M38_RS15975	10286..10903	+	618	WP_021356089.1	L,D-transpeptidase	-
C7M38_RS15980	11471..11959	+	489	WP_118131001.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18589	18589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T100925 WP_001748110.1 NZ_CP028234:7868-8212 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T100925 NZ_CP028234:7868-8212 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCGAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10142.41 Da
• Isoelectric Point: 4.8552

>AT100925 WP_160248642.1 NZ_CP028234:7611-7874 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGES
EGNELQW

Nucleotide

• Length: 264 bp

>AT100925 NZ_CP028234:7611-7874 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAGAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3