Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ImmA-IrrE/- |
| Location | 2240850..2241869 | Replicon | chromosome |
| Accession | NZ_CP008816 | ||
| Organism | Enterococcus faecalis ATCC 29212 | ||
Toxin (Protein)
| Gene name | IrrE | Uniprot ID | - |
| Locus tag | DR75_RS11595 | Protein ID | WP_002414854.1 |
| Coordinates | 2240850..2241488 (-) | Length | 213 a.a. |
Antitoxin (Protein)
| Gene name | ImmA | Uniprot ID | - |
| Locus tag | DR75_RS11600 | Protein ID | WP_002414856.1 |
| Coordinates | 2241495..2241869 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DR75_RS11565 (DR75_2156) | 2236753..2237718 | + | 966 | WP_002363484.1 | ornithine cyclodeaminase family protein | - |
| DR75_RS11570 (DR75_2157) | 2237720..2238508 | + | 789 | WP_002356143.1 | PhzF family phenazine biosynthesis protein | - |
| DR75_RS11575 (DR75_2158) | 2238557..2239102 | - | 546 | WP_002403946.1 | ATP-binding protein | - |
| DR75_RS11585 | 2239253..2239594 | - | 342 | WP_002403947.1 | hypothetical protein | - |
| DR75_RS11590 (DR75_2159) | 2240233..2240808 | + | 576 | WP_010775494.1 | restriction endonuclease | - |
| DR75_RS11595 (DR75_2160) | 2240850..2241488 | - | 639 | WP_002414854.1 | ImmA/IrrE family metallo-endopeptidase | Toxin |
| DR75_RS11600 (DR75_2161) | 2241495..2241869 | - | 375 | WP_002414856.1 | helix-turn-helix domain-containing protein | Antitoxin |
| DR75_RS16405 (DR75_2162) | 2242683..2242853 | - | 171 | WP_002414857.1 | hypothetical protein | - |
| DR75_RS11610 (DR75_2163) | 2243020..2243268 | + | 249 | WP_002414858.1 | hypothetical protein | - |
| DR75_RS11615 (DR75_2164) | 2243356..2243682 | + | 327 | WP_002365226.1 | hypothetical protein | - |
| DR75_RS11620 (DR75_2165) | 2243682..2244086 | + | 405 | WP_002414859.1 | DUF961 family protein | - |
| DR75_RS11625 (DR75_2166) | 2244240..2244476 | + | 237 | WP_010775495.1 | hypothetical protein | - |
| DR75_RS11630 (DR75_2167) | 2244479..2244883 | + | 405 | WP_002414860.1 | hypothetical protein | - |
| DR75_RS11635 (DR75_2168) | 2244995..2245327 | + | 333 | WP_002414861.1 | nucleotidyltransferase domain-containing protein | - |
| DR75_RS11640 (DR75_2169) | 2245317..2245700 | + | 384 | WP_002359294.1 | DUF86 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | - | 2224639..2291923 | 67284 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 213 a.a. Molecular weight: 25219.19 Da Isoelectric Point: 9.1721
>T10092 WP_002414854.1 NZ_CP008816:c2241488-2240850 [Enterococcus faecalis ATCC 29212]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDIPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALEYAFNTYTDFEK
MASFFNVNNIVLKIRILDELIYSHNLSYKQANLIYNGYYFQQQTQLKEILFK
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDIPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALEYAFNTYTDFEK
MASFFNVNNIVLKIRILDELIYSHNLSYKQANLIYNGYYFQQQTQLKEILFK
Download Length: 639 bp
>T10092 NZ_CP008816:c2241488-2240850 [Enterococcus faecalis ATCC 29212]
ATGATACTTAATAAGAAAAAATTATATTTTGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAATATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
ACCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTCCCTGAAGATTATCCTAAAGAGTTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAGAATATGCTTTTAACACTTATACTGATTTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACAATATAGTTCTCAAAATAAGGATTCTTGATGAATTAATTTACTCCCATAACTTATC
TTATAAACAAGCTAATCTTATTTACAATGGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTATTCAAATAA
ATGATACTTAATAAGAAAAAATTATATTTTGAAACACAGGCATTTACTGACAAACTTATAAATGAAATTTCAATCAATAT
GTCTAAAAGAAAAGAACTCATCAATTGTTATGATATTGAAGCCTACGTAAAAGAAACCGAAAATATTGATTTTTTTGATT
ATCCATTCAAAAAACAATCTAAAAAATTAATCTGGGGATCAGTAACAAAAATTGGTGATGTAACTATGGTAGCGACTGCC
CAACACTTGAAAATAGAAATTAAAAATTTTACGAAAATGCACGAAATAATTCATCATTATTTTGACTATACGACAGATAT
ACCTAACAATGAAAAATCATTTGATACACTACTTATAAAAAAGGGCTATCTCCCTGAAGATTATCCTAAAGAGTTCAGAG
CAAATAAGGGTGCCGGAATGTTACTTATTAATAAAAAAGCCTTAGAATATGCTTTTAACACTTATACTGATTTTGAAAAA
ATGGCTAGTTTTTTCAACGTAAACAATATAGTTCTCAAAATAAGGATTCTTGATGAATTAATTTACTCCCATAACTTATC
TTATAAACAAGCTAATCTTATTTACAATGGTTACTATTTTCAACAACAAACACAACTTAAAGAGATTTTATTCAAATAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 14439.25 Da Isoelectric Point: 5.0808
>AT10092 WP_002414856.1 NZ_CP008816:c2241869-2241495 [Enterococcus faecalis ATCC 29212]
METYEKIKSLRENKNITQQEVADAINMNISVYKKVELGSRPIRENELAKIADFFNVSSDYLLGRTKNPTSPQEDTTKVGE
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE
METYEKIKSLRENKNITQQEVADAINMNISVYKKVELGSRPIRENELAKIADFFNVSSDYLLGRTKNPTSPQEDTTKVGE
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE
Download Length: 375 bp
>AT10092 NZ_CP008816:c2241869-2241495 [Enterococcus faecalis ATCC 29212]
ATGGAGACTTATGAAAAAATTAAATCACTAAGAGAAAACAAAAATATTACTCAACAAGAAGTAGCCGATGCTATCAATAT
GAATATAAGTGTCTATAAAAAAGTTGAATTAGGTTCTCGACCTATAAGAGAAAATGAACTAGCAAAAATTGCAGATTTTT
TCAATGTATCTTCTGACTATTTATTGGGAAGAACTAAAAATCCTACATCCCCCCAAGAAGATACAACTAAAGTAGGAGAA
AATATTATTTCTCATTTTAGATTAAACACTAATGATTTAAGCTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA
ATGGAGACTTATGAAAAAATTAAATCACTAAGAGAAAACAAAAATATTACTCAACAAGAAGTAGCCGATGCTATCAATAT
GAATATAAGTGTCTATAAAAAAGTTGAATTAGGTTCTCGACCTATAAGAGAAAATGAACTAGCAAAAATTGCAGATTTTT
TCAATGTATCTTCTGACTATTTATTGGGAAGAACTAAAAATCCTACATCCCCCCAAGAAGATACAACTAAAGTAGGAGAA
AATATTATTTCTCATTTTAGATTAAACACTAATGATTTAAGCTCTGATGACATAGAAGAATTAGAAAAAGAATTGATTGA
TTTTCAAGAATTTCTTATAAAACGAGCAAAAGAAAGAAAAAAAAAGAGTGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Zhen Li et al. (2020) Characteristic and role of chromosomal type II toxin-antitoxin systems locus in Enterococcus faecalis ATCC29212. Journal of Microbiology (Seoul, Korea) 58(12):1027-1036. [PubMed:33095389]