Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ImmA-IrrE/- |
| Location | 1692452..1693146 | Replicon | chromosome |
| Accession | NZ_CP008816 | ||
| Organism | Enterococcus faecalis ATCC 29212 | ||
Toxin (Protein)
| Gene name | IrrE | Uniprot ID | - |
| Locus tag | DR75_RS09115 | Protein ID | WP_002370021.1 |
| Coordinates | 1692802..1693146 (+) | Length | 115 a.a. |
Antitoxin (Protein)
| Gene name | ImmA | Uniprot ID | - |
| Locus tag | DR75_RS09110 | Protein ID | WP_002370020.1 |
| Coordinates | 1692452..1692784 (+) | Length | 111 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DR75_RS09055 (DR75_1660) | 1687464..1688219 | - | 756 | WP_002414891.1 | DnaD domain protein | - |
| DR75_RS09060 (DR75_1661) | 1688234..1689049 | - | 816 | WP_002370007.1 | PD-(D/E)XK nuclease-like domain-containing protein | - |
| DR75_RS09065 (DR75_1662) | 1689012..1689983 | - | 972 | WP_002370008.1 | recombinase RecT | - |
| DR75_RS09070 (DR75_1664) | 1690034..1690288 | + | 255 | WP_002393052.1 | hypothetical protein | - |
| DR75_RS09075 (DR75_1663) | 1690263..1690481 | - | 219 | WP_002370011.1 | hypothetical protein | - |
| DR75_RS09085 (DR75_1666) | 1690585..1690809 | - | 225 | WP_002370012.1 | hypothetical protein | - |
| DR75_RS09090 (DR75_1667) | 1690806..1691105 | - | 300 | WP_002414895.1 | hypothetical protein | - |
| DR75_RS09095 (DR75_1668) | 1691192..1691608 | - | 417 | WP_002370016.1 | hypothetical protein | - |
| DR75_RS16400 (DR75_1670) | 1691802..1691945 | - | 144 | WP_002393055.1 | hypothetical protein | - |
| DR75_RS09105 (DR75_1671) | 1691956..1692132 | - | 177 | WP_002364354.1 | helix-turn-helix transcriptional regulator | - |
| DR75_RS09110 (DR75_1672) | 1692452..1692784 | + | 333 | WP_002370020.1 | helix-turn-helix domain-containing protein | Antitoxin |
| DR75_RS09115 (DR75_1673) | 1692802..1693146 | + | 345 | WP_002370021.1 | ImmA/IrrE family metallo-endopeptidase | Toxin |
| DR75_RS09120 (DR75_1674) | 1693176..1693574 | + | 399 | WP_002370022.1 | hypothetical protein | - |
| DR75_RS09125 (DR75_1675) | 1693592..1694314 | + | 723 | WP_002370023.1 | potassium channel family protein | - |
| DR75_RS09130 (DR75_1676) | 1694409..1695776 | + | 1368 | WP_002370024.1 | recombinase family protein | - |
| DR75_RS09135 (DR75_1677) | 1695798..1696373 | - | 576 | WP_002393061.1 | DNA repair protein RadC | - |
| DR75_RS09140 (DR75_1678) | 1696405..1697064 | - | 660 | WP_002385286.1 | HAD family hydrolase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1652671..1695776 | 43105 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 115 a.a. Molecular weight: 13689.61 Da Isoelectric Point: 5.5252
>T10091 WP_002370021.1 NZ_CP008816:1692802-1693146 [Enterococcus faecalis ATCC 29212]
MKSIKELVEEYEVELVFAPINKRACYEPVDRIIFVNQNLSIEEQEEAIFHEFKHVVSHSDYMELYKIPSFRNKMEAEADH
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLK
MKSIKELVEEYEVELVFAPINKRACYEPVDRIIFVNQNLSIEEQEEAIFHEFKHVVSHSDYMELYKIPSFRNKMEAEADH
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLK
Download Length: 345 bp
>T10091 NZ_CP008816:1692802-1693146 [Enterococcus faecalis ATCC 29212]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTTGCGCCAATAAATAAGCGCGCATGTTACGA
ACCAGTCGATAGGATAATTTTCGTAAATCAAAATTTATCTATTGAAGAACAAGAAGAAGCTATATTCCATGAGTTTAAAC
ATGTGGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCAGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGATGGGCAATTTAATTATTCTAATGTGATTACACATTACAATTTAAAGAT
GGGGCAGGAAACTTATTTAAAGTAA
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTTGCGCCAATAAATAAGCGCGCATGTTACGA
ACCAGTCGATAGGATAATTTTCGTAAATCAAAATTTATCTATTGAAGAACAAGAAGAAGCTATATTCCATGAGTTTAAAC
ATGTGGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCAGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGATGGGCAATTTAATTATTCTAATGTGATTACACATTACAATTTAAAGAT
GGGGCAGGAAACTTATTTAAAGTAA
Antitoxin
Download Length: 111 a.a. Molecular weight: 12777.35 Da Isoelectric Point: 4.9562
>AT10091 WP_002370020.1 NZ_CP008816:1692452-1692784 [Enterococcus faecalis ATCC 29212]
MTTFERIQELAKKRDKNLKEISIELGYSKNYLYTLKTKEPAADKLRNIANYFNVSVDYLLGRTENPNQANVNPSDDLDEV
LDNMMSFDGEPLDDHDREVIRAYLKGRFGK
MTTFERIQELAKKRDKNLKEISIELGYSKNYLYTLKTKEPAADKLRNIANYFNVSVDYLLGRTENPNQANVNPSDDLDEV
LDNMMSFDGEPLDDHDREVIRAYLKGRFGK
Download Length: 333 bp
>AT10091 NZ_CP008816:1692452-1692784 [Enterococcus faecalis ATCC 29212]
ATGACAACCTTTGAAAGGATACAAGAACTTGCAAAAAAACGTGATAAAAACTTAAAAGAGATATCAATAGAACTTGGTTA
CAGCAAAAACTACTTATATACGTTAAAAACTAAAGAGCCTGCTGCTGACAAGTTAAGAAACATTGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTAATGTTAACCCCTCTGATGATTTGGATGAAGTT
TTAGACAATATGATGAGTTTTGATGGTGAACCACTTGATGATCACGATAGAGAAGTTATTCGTGCTTATTTAAAAGGAAG
ATTCGGAAAATAA
ATGACAACCTTTGAAAGGATACAAGAACTTGCAAAAAAACGTGATAAAAACTTAAAAGAGATATCAATAGAACTTGGTTA
CAGCAAAAACTACTTATATACGTTAAAAACTAAAGAGCCTGCTGCTGACAAGTTAAGAAACATTGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTAATGTTAACCCCTCTGATGATTTGGATGAAGTT
TTAGACAATATGATGAGTTTTGATGGTGAACCACTTGATGATCACGATAGAGAAGTTATTCGTGCTTATTTAAAAGGAAG
ATTCGGAAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Zhen Li et al. (2020) Characteristic and role of chromosomal type II toxin-antitoxin systems locus in Enterococcus faecalis ATCC29212. Journal of Microbiology (Seoul, Korea) 58(12):1027-1036. [PubMed:33095389]