Detailed information of TA system TA100909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2774728..2775364
• Replicon: chromosome
• Accession: NZ_CP028215
• Organism: Bacillus subtilis strain SRCM102750

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C7M28_RS14430
• Protein ID: WP_003156187.1
• Coordinates: 2775014..2775364 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: C7M28_RS14425
• Protein ID: WP_003225183.1
• Coordinates: 2774728..2775009 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M28_RS14405	2771087..2771686	-	600	WP_032722928.1	rhomboid family intramembrane serine protease	-
C7M28_RS14410	2771781..2772146	+	366	WP_015252768.1	holo-ACP synthase	-
C7M28_RS14415	2772312..2773328	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
C7M28_RS14420	2773443..2774612	+	1170	WP_015252766.1	alanine racemase	-
C7M28_RS14425	2774728..2775009	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C7M28_RS14430	2775014..2775364	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C7M28_RS14435	2775479..2776303	+	825	WP_080320324.1	RsbT co-antagonist protein RsbRA	-
C7M28_RS14440	2776308..2776673	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
C7M28_RS14445	2776677..2777078	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
C7M28_RS14450	2777090..2778097	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
C7M28_RS14455	2778159..2778488	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
C7M28_RS14460	2778485..2778967	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
C7M28_RS14465	2778933..2779721	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
C7M28_RS14470	2779721..2780320	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T100909 WP_003156187.1 NZ_CP028215:2775014-2775364 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T100909 NZ_CP028215:2775014-2775364 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT100909 WP_003225183.1 NZ_CP028215:2774728-2775009 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT100909 NZ_CP028215:2774728-2775009 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32