Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3179274..3179910 Replicon chromosome
Accession NZ_CP028213
Organism Bacillus subtilis strain SRCM102749

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C7M27_RS16575 Protein ID WP_003156187.1
Coordinates 3179274..3179624 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag C7M27_RS16580 Protein ID WP_003225183.1
Coordinates 3179629..3179910 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M27_RS16535 3174311..3174910 - 600 WP_021481701.1 phosphoserine phosphatase RsbX -
C7M27_RS16540 3174910..3175698 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
C7M27_RS16545 3175664..3176146 - 483 WP_095010756.1 anti-sigma B factor RsbW -
C7M27_RS16550 3176143..3176472 - 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
C7M27_RS16555 3176541..3177548 - 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
C7M27_RS16560 3177560..3177961 - 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
C7M27_RS16565 3177965..3178330 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
C7M27_RS16570 3178335..3179159 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
C7M27_RS16575 3179274..3179624 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C7M27_RS16580 3179629..3179910 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C7M27_RS16585 3180026..3181195 - 1170 WP_015252766.1 alanine racemase -
C7M27_RS16590 3181310..3182326 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
C7M27_RS16595 3182492..3182857 - 366 WP_014475874.1 holo-ACP synthase -
C7M27_RS16600 3182952..3183551 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T100908 WP_003156187.1 NZ_CP028213:c3179624-3179274 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T100908 NZ_CP028213:c3179624-3179274 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT100908 WP_003225183.1 NZ_CP028213:c3179910-3179629 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT100908 NZ_CP028213:c3179910-3179629 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References