Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2310931..2311847 Replicon chromosome
Accession NZ_CP028213
Organism Bacillus subtilis strain SRCM102749

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag C7M27_RS11870 Protein ID WP_003244695.1
Coordinates 2310931..2311677 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag C7M27_RS11875 Protein ID WP_003232646.1
Coordinates 2311677..2311847 (+) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M27_RS11850 2306259..2307209 - 951 WP_015252261.1 ring-cleaving dioxygenase -
C7M27_RS11855 2307598..2308914 + 1317 WP_128740275.1 serine/threonine exchanger -
C7M27_RS11860 2309190..2309807 + 618 WP_003218470.1 DUF47 domain-containing protein -
C7M27_RS11865 2309820..2310821 + 1002 WP_070548855.1 inorganic phosphate transporter -
C7M27_RS11870 2310931..2311677 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
C7M27_RS11875 2311677..2311847 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
C7M27_RS11880 2311933..2312070 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
C7M27_RS11885 2312108..2313001 - 894 WP_021479459.1 N-acetylmuramoyl-L-alanine amidase -
C7M27_RS11890 2313014..2313277 - 264 WP_003232653.1 phage holin -
C7M27_RS11895 2313290..2313559 - 270 WP_072174140.1 hemolysin XhlA family protein -
C7M27_RS11900 2313631..2313822 - 192 WP_080375511.1 phage portal protein -
C7M27_RS11905 2313812..2314273 - 462 WP_072174134.1 hypothetical protein -
C7M27_RS11910 2314291..2315766 - 1476 WP_072174135.1 hypothetical protein -
C7M27_RS11915 2315769..2316041 - 273 WP_003232665.1 hypothetical protein -
C7M27_RS11920 2316038..2316616 - 579 WP_072174136.1 YmfQ family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T100907 WP_003244695.1 NZ_CP028213:2310931-2311677 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T100907 NZ_CP028213:2310931-2311677 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT100907 WP_003232646.1 NZ_CP028213:2311677-2311847 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT100907 NZ_CP028213:2311677-2311847 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References