Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2212251..2212887 Replicon chromosome
Accession NZ_CP028212
Organism Bacillus subtilis strain SRCM102748

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C7M26_RS11755 Protein ID WP_003156187.1
Coordinates 2212251..2212601 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag C7M26_RS11760 Protein ID WP_003225183.1
Coordinates 2212606..2212887 (-) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M26_RS11715 2207295..2207894 - 600 WP_003234303.1 phosphoserine phosphatase RsbX -
C7M26_RS11720 2207894..2208682 - 789 WP_029727187.1 RNA polymerase sigma factor SigB -
C7M26_RS11725 2208648..2209130 - 483 WP_003234299.1 anti-sigma B factor RsbW -
C7M26_RS11730 2209127..2209456 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
C7M26_RS11735 2209518..2210525 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
C7M26_RS11740 2210537..2210938 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
C7M26_RS11745 2210942..2211307 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
C7M26_RS11750 2211312..2212136 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
C7M26_RS11755 2212251..2212601 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C7M26_RS11760 2212606..2212887 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C7M26_RS11765 2213003..2214172 - 1170 WP_032722929.1 alanine racemase -
C7M26_RS11770 2214287..2215303 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
C7M26_RS11775 2215469..2215834 - 366 WP_015252768.1 holo-ACP synthase -
C7M26_RS11780 2215929..2216528 + 600 WP_032722928.1 rhomboid family intramembrane serine protease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T100896 WP_003156187.1 NZ_CP028212:c2212601-2212251 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T100896 NZ_CP028212:c2212601-2212251 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT100896 WP_003225183.1 NZ_CP028212:c2212887-2212606 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT100896 NZ_CP028212:c2212887-2212606 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References