Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 539784..540262 Replicon chromosome
Accession NC_007146
Organism Haemophilus influenzae 86-028NP

Toxin (Protein)


Gene name VapD Uniprot ID Q4QN95
Locus tag NTHI_RS02725 Protein ID WP_011272058.1
Coordinates 539784..540062 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q4QN94
Locus tag NTHI_RS02730 Protein ID WP_011272059.1
Coordinates 540071..540262 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NTHI_RS02705 (NTHI0573) 534975..536645 - 1671 WP_011272054.1 fructose-specific PTS transporter subunit EIIC -
NTHI_RS02710 (NTHI0574) 536647..537588 - 942 WP_011272055.1 1-phosphofructokinase -
NTHI_RS02715 (NTHI0575) 537590..539089 - 1500 WP_011272056.1 fused PTS fructose transporter subunit IIA/HPr protein -
NTHI_RS02720 (NTHI0576) 539161..539691 - 531 WP_005649345.1 hypothetical protein -
NTHI_RS02725 (NTHI0577) 539784..540062 - 279 WP_011272058.1 virulence-associated protein VapD Toxin
NTHI_RS02730 (NTHI0578) 540071..540262 - 192 WP_011272059.1 DUF5397 family protein Antitoxin
NTHI_RS02735 (NTHI0579) 540334..541632 - 1299 WP_005691371.1 hemolysin family protein -
NTHI_RS02740 (NTHI0580) 541683..542207 - 525 WP_011272060.1 DUF1523 family protein -
NTHI_RS02745 (NTHI0581) 542234..543016 - 783 WP_011272061.1 YchF/TatD family DNA exonuclease -
NTHI_RS02750 (NTHI0582) 543071..544054 - 984 WP_011272062.1 DNA polymerase III subunit delta' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10647.08 Da        Isoelectric Point: 4.9670

>T10089 WP_011272058.1 NC_007146:c540062-539784 [Haemophilus influenzae 86-028NP]
MYAIAFDLVVKDTQDYHPKGVQEAYTDIGAVLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRAFRI
EQWSDFTDFIRN

Download         Length: 279 bp

>T10089 NC_007146:c540062-539784 [Haemophilus influenzae 86-028NP]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAACATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7073.06 Da        Isoelectric Point: 3.8431

>AT10089 WP_011272059.1 NC_007146:c540262-540071 [Haemophilus influenzae 86-028NP]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSYLLEDPEAE

Download         Length: 192 bp

>AT10089 NC_007146:c540262-540071 [Haemophilus influenzae 86-028NP]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTAAATATCACATTGTTACAATCTGGAGAAAAAGACATTTATCGCTTAT
CTTACCTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6ZI0
PDB 6ZN8
PDB 6ZI1

Antitoxin

Source ID Structure
PDB 6ZN8
AlphaFold DB Q4QN94

References

(1) Marie B Bertelsen et al. (2021) Structural Basis for Toxin Inhibition in the VapXD Toxin-Antitoxin System. Structure (London, England : 1993) 29(2):139-150.e3. [PubMed:33096014] 3D_structure


3D_structure protein structure literature