Detailed information of TA system TA100873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3741268..3742185
• Replicon: chromosome
• Accession: NZ_CP028211
• Organism: Bacillus velezensis strain SRCM102747

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: C7M25_RS18410
• Protein ID: WP_007407256.1
• Coordinates: 3741268..3742014 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: C7M25_RS18415
• Protein ID: WP_003154807.1
• Coordinates: 3742015..3742185 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M25_RS18390	3736660..3737610	-	951	WP_012117369.1	ring-cleaving dioxygenase	-
C7M25_RS18395	3737933..3739249	+	1317	WP_160222997.1	amino acid permease	-
C7M25_RS18400	3739535..3740152	+	618	WP_003154804.1	DUF47 domain-containing protein	-
C7M25_RS18405	3740165..3741163	+	999	WP_007407255.1	inorganic phosphate transporter	-
C7M25_RS18410	3741268..3742014	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
C7M25_RS18415	3742015..3742185	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
C7M25_RS18420	3742442..3743320	-	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
C7M25_RS18425	3743334..3743597	-	264	WP_003154813.1	phage holin	-
C7M25_RS18430	3743611..3743874	-	264	WP_003154815.1	hemolysin XhlA family protein	-
C7M25_RS18435	3743926..3744687	-	762	WP_160222996.1	phage portal protein	-
C7M25_RS18440	3744744..3744941	-	198	WP_007610833.1	XkdX family protein	-
C7M25_RS18445	3744946..3745317	-	372	WP_043021266.1	YomQ/XkdW protein, phage-like element PBSX	-
C7M25_RS18450	3745330..3746961	-	1632	WP_052827325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3715088..3776078	60990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T100873 WP_007407256.1 NZ_CP028211:3741268-3742014 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T100873 NZ_CP028211:3741268-3742014 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCGCTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGATGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT100873 WP_003154807.1 NZ_CP028211:3742015-3742185 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT100873 NZ_CP028211:3742015-3742185 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14