Detailed information of TA system TA100872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 420561..421198
• Replicon: chromosome
• Accession: NZ_CP028211
• Organism: Bacillus velezensis strain SRCM102747

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C7M25_RS02175
• Protein ID: WP_003156187.1
• Coordinates: 420561..420911 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: C7M25_RS02180
• Protein ID: WP_003156188.1
• Coordinates: 420917..421198 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M25_RS02135	415601..416203	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
C7M25_RS02140	416203..416991	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
C7M25_RS02145	416957..417439	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
C7M25_RS02150	417436..417765	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
C7M25_RS02155	417829..418836	-	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
C7M25_RS02160	418848..419249	-	402	WP_003156178.1	anti-sigma regulatory factor	-
C7M25_RS02165	419252..419617	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
C7M25_RS02170	419622..420443	-	822	WP_003156182.1	STAS domain-containing protein	-
C7M25_RS02175	420561..420911	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C7M25_RS02180	420917..421198	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C7M25_RS02185	421318..422487	-	1170	WP_160222858.1	alanine racemase	-
C7M25_RS02190	422604..423611	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
C7M25_RS02195	423776..424141	-	366	WP_007410229.1	holo-ACP synthase	-
C7M25_RS02200	424234..424833	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T100872 WP_003156187.1 NZ_CP028211:c420911-420561 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T100872 NZ_CP028211:c420911-420561 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT100872 WP_003156188.1 NZ_CP028211:c421198-420917 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT100872 NZ_CP028211:c421198-420917 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5