Detailed information of TA system TA100869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3428820..3429736
• Replicon: chromosome
• Accession: NZ_CP028209
• Organism: Bacillus subtilis strain SRCM102745

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: C7M23_RS17445
• Protein ID: WP_089172519.1
• Coordinates: 3428820..3429566 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: C7M23_RS17450
• Protein ID: WP_003232646.1
• Coordinates: 3429566..3429736 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M23_RS17425	3424149..3425099	-	951	WP_003232637.1	ring-cleaving dioxygenase	-
C7M23_RS17430	3425487..3426803	+	1317	WP_015252262.1	serine/threonine exchanger	-
C7M23_RS17435	3427079..3427696	+	618	WP_003218470.1	DUF47 domain-containing protein	-
C7M23_RS17440	3427709..3428710	+	1002	WP_014479569.1	inorganic phosphate transporter	-
C7M23_RS17445	3428820..3429566	+	747	WP_089172519.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
C7M23_RS17450	3429566..3429736	+	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
C7M23_RS17455	3429822..3429974	+	153	WP_131228329.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
C7M23_RS17460	3429971..3431062	-	1092	WP_160243250.1	acyltransferase family protein	-
C7M23_RS17465	3431139..3432035	-	897	WP_131227351.1	N-acetylmuramoyl-L-alanine amidase	-
C7M23_RS17470	3432048..3432311	-	264	WP_131227350.1	phage holin	-
C7M23_RS17475	3432324..3432593	-	270	WP_015252265.1	hemolysin XhlA family protein	-
C7M23_RS17480	3432646..3433485	-	840	WP_131227349.1	phage-like element PBSX protein XepA	-
C7M23_RS17485	3433529..3433693	-	165	WP_015715740.1	XkdX family protein	-
C7M23_RS17490	3433690..3434019	-	330	WP_085186201.1	XkdW family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29121.60 Da
• Isoelectric Point: 4.6191

>T100869 WP_089172519.1 NZ_CP028209:3428820-3429566 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMFAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T100869 NZ_CP028209:3428820-3429566 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT100869 WP_003232646.1 NZ_CP028209:3429566-3429736 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT100869 NZ_CP028209:3429566-3429736 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: G4EXD8